Human papilloma virus type 57, diagnosis of HPV 57 infections and method therefor
AssigneeDade Behring Marburg GmbH
InventorsEthel-Michele de Villiers, Anja Hirsch-Behnam, Harald zur Hausen
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Abstract
The invention describes the isolation for the first time of human papilloma virus (HPV) 57, the partial characterization of its genome and its cloning in pUC 19. This opens up a way of diagnosing those tumors (oral, genital and cutaneous tumors) which are associated with HPV 57.
Claims29
A method for the diagnosis of HPV 57 infection comprising using the DNA coding for proteins, or parts of this DNA, wherein said proteins are expression products obtainable by genetic manipulation of isolate HPV 57 DNA, including equivalent variants thereto, coding for HPV 57 proteins, wherein said DNA specifically hybridizes under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
A diagnostic aid containing DNA which codes for proteins, or parts of this DNA, wherein said proteins are expression products obtainable by genetic manipulation of isolated HPV 57 DNA, including equivalent variants thereto, coding for HPV 57 proteins, wherein said DNA specifically hybridizes under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
A method for the diagnosis of HPV 57 infections, which comprises hybridizing the RNA or DNA which is to be examined with DNA which codes for the proteins which are expression products obtainable by genetic manipulation of isolated HPV 57 DNA, including equivalent variants thereto, coding for HPV 57 proteins, or for parts of these proteins, wherein said DNA or RNA specifically hybridizes under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
A method for the diagnosis of HPV 57 infections, which comprises hybridizing the DNA which is to be examined with full length HPV 57 DNA.
A method for the diagnosis of HPV 57 infections, which comprises hybridizing the DNA which is to be examined with full length DNA of HPV 57 equivalent variants.
A method for the diagnosis of HPV 57 infections, which comprises hybridizing the DNA which is to be examined with fragments of HPV 57 DNA as disclosed in FIG. 2.
HPV 57 DNA fragments as disclosed in FIG. 2.
A method for the diagnosis of HPV 57 infection comprising hybridizing the DNA which is to be examined with isolated fragments of HPV 57 DNA, wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants but not to the DNA of other HPV subtypes.
A method for the diagnosis of HPV 57 infection comprising hybridizing the DNA which is to be examined with isolated fragments of DNA of HPV 57 equivalent variants wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
HPV 57 DNA fragments, wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
DNA fragments of HPV 57 equivalent variants, wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
A method for the diagnosis of HPV 57 infections, which comprises hybridizing the DNA which is to be examined with fragments of HPV 57 DNA obtained using restriction endonucleases selected from the group consisting of Bgl II, Pvu II, Bgl I, Pst I, EcoRl, Acc I, Apa I, Kpn I, Xho I, Sac I, and Hind II, wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
HPV 57 DNA fragments having ends described by the restriction endonuclease map disclosed in FIG. 1, wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
A method for the diagnosis of HPV 57 infections, which comprises hybridizing the DNA which is to be examined with fragments of HPV 57 DNA having a length of at least 190 nucleotides, wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
HPV 57 DNA fragments having a length of at least 190 nucleotides, wherein said fragments specifically hybridize under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
A DNA fragment of deposit DSM 4694, wherein said fragment specifically hybridizes under high stringency conditions to genomic DNA selected from the group consisting of HPV 57 DNA and DNA of HPV 57 equivalent variants, but not to the DNA of other HPV subtypes.
A method for the diagnosis of HPV 57 infection comprising hybridizing the DNA which is to be examined with one or more of the fragments defined in claim 38.
Dependencies are inferred from the claim text.