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US 6,127,153 AGranted
Method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and gene encoding a polyglycosyltransferase
AssigneeNeose Technologies, Inc.
InventorsKarl F. Johnson, Stephen Roth, Stephanie L. Buczala
Life of this patent
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Abstract
The present invention relates to a method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and a gene encoding such a polyglycosyltransferase.
Claims15
A method of transferring at least two different saccharide units with a single enzyme comprising:
- (i) contacting a first activated saccharide unit with an acceptor moiety having a galactose at the non-reducing terminus, in the presence of a polyglycosyltransferase that catalyzes the linkages of said first activated saccharide unit with said acceptor moiety, to form a first saccharide composition;
- and
- (ii) contacting a second activated saccharide unit with a second saccharide composition comprising said first saccharide composition having a galactose at the non-reducing terminus, wherein said second activated saccharide unit is different from said first activated saccharide unit, in the presence of the polyglycosyltransferase, to form a preselected third saccharide composition.
A method of transferring at least two different saccharide units with a single enzyme comprising:
- (i) contacting a first activated saccharide unit with an acceptor moiety in the presence of a polyglycosyltransferase comprising the amino acid sequence of SEQ ID NO:8 that catalyzes the linkage of said first activated saccharide unit with said acceptor moiety, to form a first saccharide composition;
- and
- (ii) contacting a second activated saccharide unit with a second saccharide composition comprising said first saccharide composition, wherein said second activated saccharide unit is different from said first activated saccharide unit, in the presence of the polyglycosyltransferase, to form a preselected third saccharide composition.
A method of transferring at least two different saccharide units with a single enzyme comprising:
- (i) contacting a first activated saccharide unit with an acceptor moiety in the presence of a polyglycosyltransferase encoded by a nucleic acid hybridizable to nucleotides 445-1488 of SEQ ID NO:1 that catalyzes the linkage of said first activated saccharide unit with said acceptor moiety, to form a first saccharide composition;
- and
- (ii) contacting a second activated saccharide unit with a second saccharide composition comprising said first saccharide composition, wherein said second activated saccharide unit is different from said first activated saccharide unit, in the presence of the polyglycosyltransferase, to form a preselected third saccharide composition.
Dependencies are inferred from the claim text.