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US 6,127,141 AGranted
Culture device and method
OwnerHenry B. Kopfinventor
Life of this patent
Estimating the term…
Abstract
A culturing system and method particularly useful for producing cellular products such as viral pathogens of cells. It includes a mass transfer culture segment, stacked filter plates to adjust the medium composition, and a product removal and concentration segment. The mass transfer culture segment utilizes changed directional flow of the medium to maximize cell growth and production of product. The stacked filter plates allow addition of sterile fresh medium and removal of growth inhibitory substances.
Claims56
A mass transfer system comprising:
- (a) a first tangential flow membrane device comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into two or more chambers, including a first set of chambers comprising an intra-filter volume and a second set of chambers comprising an extra-filter volume;
- (b) a second tangential flow membrane device in fluid communication with one or more of the chambers of the first tangential flow membrane device, said second tangential flow membrane device comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into two or more chambers, including a first set of chambers comprising an intra-filter volume and a second set of chambers comprising an extra-filter volume;
- (c) one or more substance addition means in fluid communication with the extra-filter volume or intra-filter volume of the first and/or second tangential flow membrane device;
- and
- (d) one or more fluid exit means in fluid communication with the extra-filter volume or intra-filter volume of the first and/or second tangential flow device.
6 more elements
A mass transfer system comprising:
- (a) a first tangential flow membrane device comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (b) one or more substance addition means in fluid communication with one or more of the chambers of the first and or second tangential flow membrane device;
- (c) a second tangential flow membrane device in fluid communication with one or more of the chambers of the first and/or second tangential flow membrane device, said second tangential flow membrane device comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers;
- and
- (d) a fluid exit means in fluid communication with the first and or second tangential flow membrane device;
- wherein a first set of one or more chambers of the first tangential flow membrane device are connected in loop fluid communication with a first set of one or more chambers of the second tangential flow membrane device such that fluid exiting the first set of one or more chambers of the first tangential flow membrane device flows into the first set of one or more chambers of the second tangential flow membrane device, and fluid exiting first set of one or more chambers of the second tangential flow membrane device flows into the first set of one or more chambers of the first tangential flow membrane device.
7 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device in fluid connection with the medium reservoir comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system operationally positioned in the path of fluid communication between the reservoir and the tangential flow growth device, for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a sterile barrier tangential flow membrane device connected in fluid communication with one or more of the extra-filter chambers or the intra filter chambers of the tangential flow growth device comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers;
- and
- (e) a means for monitoring medium conditions in the medium reservoir.
7 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device connected to the medium reservoir comprising:
- i) a fist housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system having discharge and inlet ports for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a sterile barrier tangential flow membrane device connected in fluid communication with one or more of the extra-filter chambers or the intra filter chambers of the tangential flow growth device comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers;
- and
- (e) a means for monitoring medium conditions in the medium reservoir;
- and
- (f) a tangential flow filtering device in fluid communication with the medium reservoir for concentrating and/or dialysis of the medium.
9 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device connected to the medium reservoir comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system having discharge and inlet ports for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a sterile barrier membrane device connected in fluid communication with one or more or the extra-filter chambers of the tangential flow growth device comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers;
- and
- (e) a means for monitoring medium conditions.
7 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device connected in fluid communication with the medium reservoir comprising:
- i) a housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system, operationally interposed in a path of fluid communication between the reservoir and the tangential flow growth device, for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a means for monitoring medium conditions;
- and
- (e) an auxiliary flow circuit comprising an auxiliary pump, joined in fluid communication with the tangential flow growth device and arranged for flowing cell culture from the tangential flow growth device through the auxiliary circuit for recirculation thereof.
5 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device connected in fluid communication with the medium reservoir comprising:
- i) a housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system operationally interposed in a path of fluid communication between the reservoir and the tangential flow growth device, for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a means for monitoring medium conditions;
- and
- (e) an auxiliary flow circuit comprising an auxiliary pump and auxiliary reservoir, joined in fluid communication with the tangential flow growth device and arranged for flowing cell culture from the tangential flow growth device through the auxiliary circuit for recirculation thereof.
5 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more hollow fiber filters separating the interior volume of the first housing into one or more extra-fiber chambers and one or more intra-fiber chambers;
- (c) a pump system operationally positioned in a path of fluid communication between the reservoir and the tangential flow growth device for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a sterile barrier membrane device connected in fluid communication with one or more of the extra-fiber chambers of the tangential flow growth device comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers;
- and
- (e) a means for monitoring medium conditions;
- and
- (f) a tangential flow filter device arranged in fluid communication with the medium reservoir for concentrating and/or dialysis of the medium.
9 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a sterile barrier tangential flow membrane device comprising;
- i) a housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system operationally positioned in a path of fluid communication between the reservoir and the tangential flow growth device for pumping the medium from the reservoir to the sterile barrier tangential flow membrane device;
- and
- (d) a means for monitoring medium conditions.
4 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a sterile barrier tangential flow membrane device comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system operationally positioned in a path of fluid communication between the reservoir and the tangential flow growth device for pumping the medium from the reservoir to the sterile barrier tangential flow membrane device;
- (d) a means for monitoring medium conditions;
- and
- (e) a tangential flow filter device for concentrating and/or dialysis of the medium removed via the sterile barrier tangential flow membrane device comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers.
7 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device connected to the medium reservoir comprising:
- i) a housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system operationally positioned in a path of fluid communication between the reservoir and one or more chambers of the tangential flow growth device for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a means for circulating the fluid in the extra-capillary space of the tangential flow culture device;
- and
- (e) means for monitoring medium conditions.
5 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device connected to the medium reservoir comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system operationally positioned in a path of fluid communication between the reservoir and the tangential flow growth device for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a means for circulating the fluid in the extra-capillary space of the tangential flow culture device;
- (e) a reservoir for the fluid in the extra-capillary space of the tangential flow culture device;
- (f) a means of removing the fluid in the extra-capillary space;
- (g) a means for monitoring medium conditions;
- and
- (h) a tangential flow filter device in fluid communication with the medium reservoir for concentrating and/or dialysis of the medium comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers.
10 more elements
A mass transfer system, comprising:
- (a) a medium reservoir containing a growth medium;
- (b) a tangential flow growth device connected to the medium reservoir comprising:
- i) a first housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the first housing into one or more extra-filter chambers and one or more intra-filter chambers;
- (c) a pump system operationally positioned in a path of fluid communication between the reservoir and the tangential flow growth device for pumping the medium from the reservoir to the tangential flow growth device;
- (d) a means for circulating the fluid in the extra-capillary space of the tangential flow culture device;
- (e) a reservoir for the fluid and cells in the extra-capillary space of the tangential flow culture device;
- (f) a means of removing fluid from the extra-capillary space that includes a sterile barrier filter a means for monitoring medium conditions;
- and
- (g) a tangential flow filter device in fluid communication with the medium reservoir for concentration and/or dialysis of the medium comprising:
- i) a second housing enclosing an interior volume;
- ii) one or more filter means separating the interior volume of the second housing into one or more extra-filter chambers and one or more intra-filter chambers.
9 more elements
Dependencies are inferred from the claim text.