Method of enzymatically measuring glycated protein
AssigneeKyoto Daiichi Kagaku Co., Ltd.
InventorsKaori Ishimaru, Katsutaka Ooishi, Hiroshi Fukuya, Akihiko Okamura
Life of this patent
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Abstract
A sample containing a glycated protein is treated with Protease XIV or a protease from Aspergillus genus, thereafter (or while treating the sample with the above protease) FAOD (fructosyl amino acid oxidase) is caused to react with the sample so as to measure the amount of oxygen consumed by the FAOD reaction or the amount of the resultant reaction product, thereby to measure the glycated protein. According to the above method, the glycated protein can be fragmented while the decomposition of the FAOD itself is prevented, thereby to facilitate the binding of the protein with the FAOD and to improve the sensitivity of the detection.
Claims18
A protease to be used for measuring a glycated protein in a sample in combination with FAOD (fructosyl amino acid oxidase), which protease is obtained from the Aspergillus genus.
A method of measuring a glycated protein by causing FAOD to act on a sample containing a glycated protein, wherein the glycated protein is treated with a protease from the Aspergillus genus under an acid condition.
A method of measuring a glycated protein by causing protease and FAOD to act on a sample containing a glycated protein, wherein a protease from Aspergillus melleus (A. melleus) is used as the protease.
A method of measuring a glycated protein by causing protease and FAOD to act on a sample containing a glycated protein, wherein Protease XIV is the protease.
A method of measuring a glycated protein by causing FAOD to act on a sample containing a glycated protein, wherein the glycated protein is treated with a protease from Aspergillus genus under an alkaline condition.
Dependencies are inferred from the claim text.