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US 6,127,119 AGranted
Nucleic acid ligands of tissue target
AssigneeNeXstar Pharmaceuticals, Inc.
InventorsAndrew Stephens, Larry Gold, Ulrich Speck
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Abstract
This invention discloses high-affinity oligonucleotide ligands to complex tissue targets, specifically nucleic acid ligands having the ability to bind to complex tissue targets, and the methods for obtaining such ligands. Tissue targets comprise cells, subcellular components, aggregates or cells, collections of cells, and higher ordered structures. Specifically, nucleic acid ligands to blood vessels are described.
Claims21
A method for identifying a nucleic acid ligand to a blood vessel comprising:
- a) preparing a candidate mixture of nucleic acid sequences;
- b) contacting said candidate mixture of nucleic acids with said blood vessel, wherein nucleic acids having an increased affinity to the blood vessel relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- and
- d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to said blood vessel, whereby a nucleic acid ligand of said blood vessel may be identified.
2 more elements
A purified and isolated non-naturally occurring nucleic acid ligand to a blood vessel.
Dependencies are inferred from the claim text.