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US 6,127,116 AGranted
Functional DNA clone for hepatitis C virus (HCV) and uses thereof
AssigneeWashington University
InventorsCharles M. Rice, Alexander A. Kolykhalov
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Abstract
The present invention relates to the determination of an authentic HCV genome RNA sequences, to construction of infectious HCV DNA clones, and to use of the clones, or their derivatives, in therapeutic, vaccine, and diagnostic applications. The invention is also directed to HCV vectors, e.g., for gene therapy of gene vaccines.
Claims9
An HCV double-stranded cDNA which can be transcribed to produce replicating HCV RNA transcripts, wherein the cDNA comprises:
- (a) a 5' terminus selected from the group consisting of GCCAGCC, GGCCAGCC, UGCCAGCC, AGCCAGCC, AAGCCAGCC, GAGCCAGCC, GUGCCAGCC, and GCGCCAGCC, wherein nucleotides 8-17 of SEQ ID NO:3 are immediately 3' to the 5' terminus;
- (b) an HCV polyprotein coding region;
- (c) a 3' terminus consisting of a nucleotide sequence selected from the group consisting of SEQ ID NO:4;
- SEQ ID NO:4 wherein the nucleotides at positions 58, 78, 93, and 99 are W;
- SEQ ID NO:4 wherein the nucleotides at positions 58, 78, 93, and 99 are W and CTCTCT are inserted between the nucleotides at positions 90 and 91;
- and the first 39 nucleotides of SEQ ID NO:4.
3 more elements
A diagnostic test kit for HCV comprising a component derived from an authentic HCV virus 3'NTR, wherein said component comprises SEQ ID NO:4.
Dependencies are inferred from the claim text.