Baculovirus expression vectors and recombinant antigens for detecting type-specific antibodies to herpes simplex virus
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsPhilip E. Pellett, Demetrio Sanchez-Martinez
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Abstract
Novel baculovirus expression vectors and recombinant antigens for detecting, type-specific herpes simplex virus (HSV) infection have been made. Diagnostic kits and assays for detecting type-specific HSV infection have been described. High level production of foreign proteins in substantially pure form are now made possible by the novel baculoviruses of the present invention.
Claims8
Recombinant herpes simplex virus gG-1 antigen produced by employing a recombinant baculovirus having a nontranslated polyhedrin gene leader sequence CTATAAAT (SEQ ID NO. 7) joined to the 5' end of a polyhedrin gene translation initiation codon ATG, and having the polyhedrin gene translation initiation codon ATG joined to the 5' end of the coding region of a foreign gene, without any extraneous nucleotide between the 5' end of the nontranslated polyhedrin gene leader sequence CTATAAAT (SEQ ID NO. 7), the polyhedrin gene translation initiation codon ATG and the 5' end of the coding region of a foreign gene, wherein said foreign gene is herpes simplex virus type 1 glycoprotein gene.
Recombinant herpes simplex virus gG-2 antigen produced by employing a recombinant baculovirus having a nontranslated polyhedrin gene leader sequence CTATAAAT (SEQ ID NO. 7) joined to the 5' end of a polyhedrin gene translation initiation codon ATG, and having the polyhedrin gene translation initiation codon ATG joined to the 5' end of the coding region of a foreign gene, without any extraneous nucleotide between the 5' end of the nontranslated polyhedrin gene leader sequence CTATAAAT (SEQ ID NO. 7), the polyhedrin gene translation initiation codon ATG and the 5' end of the coding region of a foreign gene, wherein said foreign gene is herpes simplex virus type 2 glycoprotein gene.
A composition comprising purified recombinant baculovirus expressed herpes simplex virus gG-1 antigen or herpes simplex virus gG-2 antigen in a pharmaceutically acceptable carrier, wherein the recombinant baculovirus has a nontranslated polyhedrin gene leader sequence CTATAAAT (SEQ ID NO. 7) joined to the 5' end of a polyhedrin gene translation initiation codon ATG, and has the polyhedrin gene translation initiation codon ATG joined to the 5' end of the coding region of the herpes simplex virus type 1 glycoprotein gene or the herpes simplex virus type 2 glycoprotein gene, without any extraneous nucleotide between the 5' end of the nontranslated polyhedrin gene leader sequence CTATAAAT (SEQ ID NO. 7), the polyhedrin gene translation initiation codon ATG and the 5' end of the coding region of the herpes simplex virus type 1 glycoprotein gene or the herpes simplex virus type 2 glycoprotein gene.
Dependencies are inferred from the claim text.