Surface-enhanced photolabile attachment and release for desorption and detection of analytes
AssigneeBaylor College of Medicine
InventorsT. William Hutchens, Tai-Tung Yip
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Abstract
This invention is directed to systems for presenting an analyte to an energy source for desorption in methods of analytic detection, such as mass spectrometry. The probes have a layer of energy absorbing molecules on their surface which absorbs energy form the energy source, thereby enabling desorption of the analyte form the probe surface. The layer of energy absorbing molecules is immobilized by chemical bonding to the probe surface and/or is substantially free of crystals.
Claims61
A system for detecting an analyte comprising:
- a removably insertable probe having a surface for presenting the analyte to an energy source that emits energy capable of desorbing the analyte from the probe surface, and a layer of energy absorbing molecules immobilized by chemical bonding to the surface, the energy absorbing molecules being capable of absorbing the energy and enabling desorption of the analyte;
- an energy source that directs energy to the probe surface for desorbing the analyte;
- and
- a detector in communication with the probe surface that detects the desorbed analyte.
A method for detecting an analyte comprising the steps of:
- a) providing a system comprising:
- (1) a removably insertable probe having a surface for presenting the analyte to an energy source that emits energy capable of desorbing the analyte from the probe surface, and a layer of energy absorbing molecules immobilized by chemical bonding to the surface, the energy absorbing molecules being capable of absorbing the energy and enabling desorption of the analyte;
- (2) an energy source that directs energy to the probe surface for desorbing the analyte;
- and
- (3) a detector in communication with the probe surface that detects the desorbed analyte;
- b) desorbing at least a portion of the analyte from the probe surface by exposing the analyte to the energy;
- and
- c) detecting the desorbed analyte with the detector.
A system for detecting an analyte comprising:
- a removably insertable probe having a surface for presenting the analyte to an energy source capable of desorbing the analyte from the probe, and a layer of energy absorbing molecules on the surface, the layer substantially free of crystals, the energy absorbing molecules being capable of absorbing the energy and enabling desorption of the analyte;
- an energy source that emits energy that desorbs the analyte from the probe surface;
- and
- a detector that detects the desorbed analyte.
A method for detecting an analyte comprising the steps of:
- a) providing a system comprising:
- (1) a removably insertable probe having a surface for presenting the analyte to an energy source capable of desorbing the analyte from the probe, and a layer of energy absorbing molecules on the surface, the layer substantially free of crystals, the energy absorbing molecules being capable of absorbing the energy and enabling desorption of the analyte;
- (2) an energy source that emits energy that desorbs the analyte from the probe surface;
- and
- (3) a detector that detects the desorbed analyte;
- b) desorbing at least a portion of the analyte from the probe surface by exposing the analyte to the energy;
- and
- c) detecting the desorbed analyte with the detector.
Dependencies are inferred from the claim text.