Endo-xyloglucan transferase
AssigneeTakara Shuzo Co., Ltd.
InventorsKazuhiko Nishitani, Kazuhide Okazawa, Kiyozo Asada, Ikunoshin Kato
Life of this patent
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Abstract
Endo-xyloglucan transferases responsible for growth of plant cell wall, genes coding for the enzymes, a method of transferring xyloglucan molecules by using the enzyme, and methods of using the gene are described.
Claims28
A method for inhibiting the expression of an endo-xyloglucan transferase in a plant which produces endo-xyloglucan transferase, said method comprising introducing into the plant a heterologous expression vector for forming an antisense RNA, wherein the heterologous expression vector comprises a DNA comprising the sequence shown in SEQ ID NO. 15 which codes for an endo-xyloglucan transferase in a 5'-3' direction, said DNA being operably linked in a reverse 3'-5' direction downstream of a promoter.
A method for inhibiting the expression of an endo-xyloglucan transferase in a plant which produces endo-xyloglucan transferase, said method comprising introducing into the plant a heterologous expression vector for forming an antisense RNA, wherein the heterologous expression vector comprises a DNA which codes for a protein in a 5'-3' direction having endo-xyloglucan transferase activity, wherein the complement of said DNA hybridizes with the sequence shown in SEQ ID NO. 15 under the following conditions:
- solution:
- 5.times.Denhart's solution, 6.times.SSC, 0.1% SDS, 10 .mu.g/ml salmon sperm DNA,
- hybridization:
- incubating at 50.degree. C. overnight,
- said DNA being operably linked in a reverse 3'-5' direction downstream of a promoter.
A method for regulating the morphology of a plant, said method comprising introducing into the plant a heterologous expression vector for forming an antisense RNA, wherein the heterologous expression vector comprises a DNA comprising the sequence shown in SEQ ID NO. 15 which codes for an endo-xyloglucan transferase in a 5'-3' direction, said DNA being operably linked in a reverse 3'-5' direction downstream of a promoter, to thereby regulate the morphology of the plant by controlling the expression of an endo-xyloglucan transferase in the plant.
A method for regulating the morphology of a plant, said method comprising introducing into the plant a heterologous expression vector for forming an antisense RNA, wherein the heterologous expression vector comprises a DNA which codes for a protein in a 5'-3' direction having endo-xyloglucan transferase activity, wherein the complement of said DNA hybridizes with the sequence shown in SEQ ID NO. 15 under the following conditions:
- solution:
- 5.times.Denhart's solution, 6.times.SSC, 0.1% SDS, 10 .mu.g/ml salmon sperm DNA,
- hybridization:
- incubating at 50.degree. C. overnight,
- said DNA being operably linked in a reverse 3'-5' direction downstream of a promoter, to thereby regulate the morphology of the plant by controlling the expression of an endo-xyloglucan transferase in the plant.
A plant having a heterologous expression vector for forming an antisense RNA introduced into the plant, wherein the heterologous expression vector comprises a DNA comprising the sequence shown in SEQ ID NO. 15 which codes for an endo-xyloglucan transferase in a 5'-3' direction, said DNA being operably linked in a reverse 3'-5' direction downstream of a promoter.
A plant having a heterologous expression vector for forming an antisense RNA introduced into the plant, wherein the heterologous expression vector comprises a DNA which codes for a protein in a 5'-3' direction having endo-xyloglucan transferase activity, wherein the complement of said DNA hybridizes with the sequence shown in SEQ ID NO. 15 under the following conditions:
- solution:
- 5.times.Denhart's solution, 6.times.SSC, 0.1% SDS, 10 .mu.g/ml salmon sperm DNA,
- hybridization:
- incubating at 50.degree. C. overnight,
- said DNA being operably linked in a reverse 3'-5' direction downstream of a promoter.
A method for expressing an endo-xyloglucan transferase in a plant, said method comprising introducing into the plant a heterologous expression vector, wherein the heterologous expression vector comprises a DNA comprising the sequence shown in SEQ ID NO. 15 which codes for an endo-xyloglucan transferase in a 5'-3' direction, said DNA being operably linked in a 5'-3' direction downstream of a promoter.
A method for expressing an endo-xyloglucan transferase in a plant, said method comprising introducing into the plant a heterologous expression vector, wherein the heterologous expression vector comprises a DNA which codes for a protein in a 5'-3' direction having endo-xyloglucan transferase activity, wherein the complement of said DNA hybridizes with the sequence shown in SEQ ID NO. 15 under the following conditions:
- solution:
- 5.times.Denhart's Solution, 6.times.SSC, 0.1% SDS, 10 .mu.g/ml salmon sperm DNA,
- hybridization:
- incubating at 50.degree. C. overnight,
- said DNA being operably linked in a 5'-3' direction downstream of a promoter.
A method for regulating the morphology of a plant, said method comprising introducing into the plant a heterologous expression vector, wherein the heterologous expression vector comprises a DNA comprising the sequence shown in SEQ ID NO. 15 which codes for an endo-xyloglucan transferase in a 5'-3' direction, said DNA being operably linked in a 5'-3' direction downstream of a promoter, to thereby regulate the morphology of the plant by expressing an endo-xyloglucan transferase in the plant.
A method for regulating the morphology of a plant, said method comprising introducing into the plant a heterologous expression vector, wherein the heterologous expression vector comprises a DNA which codes for a protein in a 5'-3' direction having endo-xyloglucan transferase activity, wherein the complement of said DNA hybridizes with the sequence shown in SEQ ID NO. 15 under the following conditions:
- solution:
- 5.times.Denhart's solution, 6.times.SSC, 0.1% SDS, 10 .mu.g/ml salmon sperm DNA,
- hybridization:
- incubating at 50.degree. C. overnight,
- said DNA being operably linked in a 5'-3' direction downstream of a promoter, to thereby regulate the morphology of the plant by expressing an endo-xyloglucan transferase in the plant.
A plant having a heterologous expression vector introduced into the plant, wherein the heterologous expression vector comprises a DNA comprising the sequence shown in SEQ ID NO. 15 which codes for an endo-xyloglucan transferase in a 5'-3' direction, said DNA being operably linked in a 5'-3' direction downstream of a promoter.
A plant having a heterologous expression vector introduced into the plant, wherein the heterologous expression vector comprises a DNA which codes for a protein in a 5'-3' direction having endo-xyloglucan transferase activity, wherein the complement of said DNA hybridizes with the sequence shown in SEQ ID NO. 15 under the following conditions:
- solution:
- 5.times.Denhart's solution, 6.times.SSC, 0.1% SDS, 10 .mu.g/ml salmon sperm DNA,
- hybridization:
- incubating at 50.degree. C. overnight,
- said DNA being operably linked in a 5'-3' direction downstream of a promoter.
A method for producing an endo-xyloglucan transferase, said method comprising, incubating a plant or cells transformed with a DNA coding for an endo-xyloglucan transferase to express the endo-xyloglucan in the plant or cells, wherein the DNA comprises the sequence shown in SEQ ID NO. 15, and recovering the endo-xyloglucan transferase from the culture.
A method for producing an endo-xyloglucan transferase, said method comprising incubating a plant or cells transformed with a DNA coding for an endo-xyloglucan transferase to express the endo-xyloglucan in the plant or cells, wherein the DNA comprises a DNA sequence whose complement hybridizes with the sequence shown in SEQ ID NO. 15 under the following conditions:
- solution:
- 5.times.Denhart's solution, 6.times.SSC, 0.1% SDS, 10 .mu.g/ml salmon sperm DNA
- hybridization:
- incubating at 50.degree. C. overnight,
- and recovering the endo-xyloglucan transferase from the culture.
Dependencies are inferred from the claim text.