Mobilization of peripheral blood precursor cells by .beta.(1,3)-glucan
AssigneeThe Collaborative Group, Ltd.
InventorsMyra L. Patchen, Paul Bleicher
Life of this patent
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Abstract
Methods for inducing mobilization of peripheral blood precursor cells, for elevating levels of peripheral blood precursor cells and facilitating hematopoietic reconstitution in a mammal are disclosed, comprising administering to the mammal an effective amount of an underivatized, aqueous soluble .beta.(1,3)-glucan in a triple helix conformation, which does not stimulate or prime the production of proinflammatory cytokines in vitro.
Claims22
A method for facilitating hematopoietic reconstitution of peripheral blood precursor cells in a mammal's hematopoietic organs, comprising:
- a) administering to the mammal an effective amount of an underivatized, aqueous soluble .beta.(1,3)-glucan in a triple helix conformation that is sufficient to cause the peripheral blood precursor cells to mobilize from hematopoietic organs into the mammal's peripheral circulation, thereby elevating the level of circulating peripheral blood precursor cells, wherein the .beta.(1,3)-glucan does not stimulate or prime the production of proinflammatory cytokines, in vitro or in vivo;
- b) isolating mobilized peripheral blood precursor cells from the peripheral circulation of the mammal;
- and
- c) infusing the isolated mobilized peripheral blood precursor cells into the mammal.
A method for inducing mobilization of hematopoietic progenitor cells in a mammal, comprising:
- a) administering to the mammal an effective amount of an underivatized aqueous soluble .beta.(1,3)-glucan in a triple helix conformation that is sufficient to induce mobilization of hematopoietic progenitor cells from hematopoietic organs into the mammal's peripheral circulation;
- b) isolating mobilized hematopoietic progenitor cells from the mammal;
- and
- c) infusing the isolated mobilized hematopoietic progenitor cells into a mammal;
- wherein the .beta.(1,3)-glucan does not stimulate or prime the production of proinflammatory cytokines, in vitro or in vivo.
A method for obtaining a therapeutically effective level of peripheral blood precursor cells, comprising:
- a) administering to the mammal an effective amount of an underivatized, aqueous soluble .beta.(1,3)-glucan in a triple helix conformation that is sufficient to cause the peripheral blood precursor cells to mobilize from hematopoietic organs into mammal's peripheral circulation, wherein the .beta.(1,3)-glucan does not stimulate or prime the production of proinflammatory cytokines, in vitro or in vivo;
- and
- b) isolating mobilized peripheral blood precursor cells from the peripheral circulation of the mammal to obtain a therapeutically effective level of said cells.
A method for obtaining a therapeutically effective level of ex vivo expanded cells from a population of peripheral blood precursor cells, comprising:
- a) administering to the mammal an effective amount of an underivatized, aqueous soluble .beta.(1,3)-glucan in a triple helix conformation that is sufficient to cause the peripheral blood precursor cells to mobilize from the hematopoietic organs into the mammal's peripheral circulation, wherein the .beta.(1,3)-glucan does not stimulate or prime the production of proinflammatory cytokines, in vitro or in vivo;
- b) isolating mobilized peripheral blood precursor cells from the peripheral circulation of the mammal;
- and
- c) causing the isolated mobilized peripheral blood precursor cell population to expand to thereby obtain a therapeutically effective level of said cells.
A method for obtaining a therapeutically effective level of peripheral blood precursor cells, comprising:
- a) administering to the mammal an effective amount of a growth factor which induces mobilization of peripheral blood precursor cells and an underivatized, aqueous soluble .beta.(1,3)-glucan in a triple helix conformation that is sufficient to cause the peripheral blood precursor cells to mobilize from hematopoietic organs into the mammal's peripheral circulation, wherein the .beta.(1,3)-glucan does not stimulate or prime the production of proinflammatory cytokines, in vitro or in vivo;
- and
- b) isolating mobilized peripheral blood precursor cells from the peripheral circulation of the mammal to obtain a therapeutically effective level of said cells.
A method for obtaining a therapeutically effective level of ex vivo expanded cells from a population of peripheral blood precursor cells, comprising:
- a) administering to the mammal an effective amount of a growth factor which induces immobilization of peripheral blood precursor cells and an underivatized, aqueous soluble .beta.(1,3)-glucan in a triple helix conformation that is sufficient to cause the peripheral blood precursor cells to mobilize from hematopoietic organs into the mammal's peripheral circulation, wherein the .beta.(1,3)-glucan does not stimulate or prime the production of proinflammatory cytokines, in vitro or in vivo;
- b) isolating mobilized peripheral blood precursor cells from the peripheral circulation of the mammal;
- and
- c) causing the isolated mobilized peripheral blood precursor cell population to expand to thereby obtain a therapeutically effective level of said cells.
Dependencies are inferred from the claim text.