Expression vectors
AssigneeNeose Technologies, Inc.
InventorsJody Schultz, Gary Hermanson
Life of this patent
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Abstract
The present invention provides isolated recombinant nucleic acid constructs comprising a dual bacterial promoter operably linked to a heterologous nucleic acid which encodes a desired polypeptide. The constructs are useful for the expression of the desired polypeptides in bacterial cells at high levels.
Claims48
A recombinant nucleic acid construct comprising a dual bacterial promoter operably linked to a heterologous nucleic acid that encodes a desired polypeptide, wherein the dual bacterial promoter comprises a tac promoter component and a gal promoter component.
An expression vector which comprises a selectable marker and a recombinant nucleic acid construct comprising a dual bacterial promoter operably linked to a heterologous nucleic acid that encodes a desired polypeptide, wherein the dual bacterial promoter comprises a tac promoter component and a gal promoter component.
A plasmid which is identical to a plasmid deposited with the American Type Culture Collection under Accession No. 98059.
A recombinant nucleic acid construct comprising a Streptococcus thermophilus UDPglucose-4-epimerase promoter operably linked to a heterologous nucleic acid that encodes a desired polypeptide.
A bacterial cell comprising a recombinant expression cassette comprising a dual bacterial promoter operably linked to a heterologous nucleic acid that encodes a desired polypeptide, wherein the dual bacterial promoter comprises a tac promoter component and a gal promoter component.
A method of making a desired polypeptide, the method comprising culturing in an appropriate medium bacterial cells comprising a recombinant expression cassette comprising a dual bacterial promoter operably linked to a heterologous nucleic acid that encodes a desired polypeptide under conditions that allow expression of the desired polypeptide, wherein the dual bacterial promoter comprises a tac promoter component and a gal promoter component.
Dependencies are inferred from the claim text.