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AssigneeIntergen Company
InventorsIrina A. Nazarenko, Satish K. Bhatnagar, Emily S. Winn-Deen, Robert J. Hohman
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The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluorophores, such that fluorescent energy emitted by the donor is absorbed by the acceptor. The acceptor may be a fluorophore that fluoresces at a wavelength different from the donor moiety, or it may be a quencher. The oligonucleotides of the invention are configured so that a donor moiety and an acceptor moiety are incorporated into the amplification product. The invention also provides methods and kits for directly detecting amplification products employing the nucleic acid amplification primers. When labeled linear primers are used, treatment with exonuclease or by using specific temperature eliminates the need for separation of unincorporated primers. This "closed-tube" format greatly reduces the possibility of carryover contamination with amplification products, provides for high throughput of samples, and may be totally automated.
An oligodeoxynucleotide, the sequence of which consists of:
An oligodeoxynucleotide, the sequence of which consists of:
An oligodeoxynucleotide, the sequence of which consists of:
An oligodeoxynucleotide, the sequence of which consists of:
An oligodeoxynucleotide, the sequence of which consists of:
An oligonucleotide containing:
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:1, wherein fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:1, and DABCYL is attached to the nucleotide at position 22 of SEQ ID NO:1.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:1, wherein a derivative of fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:1, and DABCYL is attached to the nucleotide at position 22 of SEQ ID NO:1.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:13, wherein fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:13, and DABCYL is attached to the nucleotide at position 20 of SEQ ID NO:13.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:13, wherein a derivative of fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:13, and DABCYL is attached to the nucleotide at position 20 of SEQ ID NO:13.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:14, wherein fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:14, and DABCYL is attached to the nucleotide at position 24 of SEQ ID NO:14.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:14, wherein a derivative of fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:14, and DABCYL is attached to the nucleotide at position 24 of SEQ ID NO:14.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:15, wherein fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:15, and DABCYL is attached to the nucleotide at position 24 of SEQ ID NO:15.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:15, wherein a derivative of fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:15, and DABCYL is attached to the nucleotide at position 24 of SEQ ID NO:15.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:16, wherein fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:16, and DABCYL is attached to the nucleotide at position 22 of SEQ ID NO:16.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:16, wherein a derivative of fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:16, and DABCYL is attached to the nucleotide at position 22 of SEQ ID NO:16.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:17, wherein fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:17, and DABCYL is attached to the nucleotide at position 22 of SEQ ID NO:17.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:17, wherein a derivative of fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:17, and DABCYL is attached to the nucleotide at position 22 of SEQ ID NO:17.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:18, wherein fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:18, and DABCYL is attached to the nucleotide at position 20 of SEQ ID NO:18.
An oligonucleotide comprising the nucleotide sequence of SEQ ID NO:18, wherein a derivative of fluorescein is attached to the nucleotide at position 1 of SEQ ID NO:18, and DABCYL is attached to the nucleotide at position 20 of SEQ ID NO:18.
A kit comprising:
A kit comprising first and second oligonucleotides,
A kit comprising:
Dependencies are inferred from the claim text.