Supplemented and unsupplemented tissue sealants, method of their production and use

A biocompatible, supplemented tissue sealant composition comprising:

• (i) at least one supplement selected from the group consisting of an antiangiogenin, an antifibrinolytic protein, an antimicrobial protein, a cytokine, a hormone, an interferon, a proteoglycan, a polypeptide, a protease inhibitor, a cytotoxin or cell proliferation inhibiting protein, and a protein nutritional supplement;
• and
• (ii) fibrinogen in an amount which forms a fibrin matrix;
• wherein said fibrinogen will form a fibrin matrix when in the presence of thrombin and Ca.sup.++ and water,
• further wherein said supplement is released from said fibrin matrix into the external environment of use for a sustained period, and
• further wherein said sustained period is greater than the period obtained according to simple diffusion kinetics.

A biocompatible, supplemented tissue sealant composition comprising:

• (i) at least one supplement selected from the group consisting of a growth factor, an osteogenic protein, a cartilage inducing protein, an antibody, and an anticoagulant protein;
• and
• (ii) fibrinogen in an amount which forms a fibrin matrix;
• wherein said fibrinogen will form a fibrin matrix when in the presence of thrombin and Ca.sup.++ and water,
• further wherein said supplement is released from said fibrin matrix into the external environment of use for a sustained period, and
• further wherein said sustained period is greater than the period obtained according to simple diffusion kinetics.