Method for modulating smooth muscle cell proliferation
AssigneeArch Development Corporation
InventorsMichael S. Parmacek, Julian Solway
Life of this patent
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Abstract
Disclosed is a smooth muscle cell specific promoter, the SM22.alpha. gene promoter as well as the murine cDNA and genomic SM22.alpha. nucleic acid sequences. Also disclosed are methods of preventing restenosis following balloon angioplasty and methods of treating asthma based on inhibition of smooth muscle cell proliferation by expressing cell cycle control genes, or contraction inhibiting peptides in smooth muscle cells, under the control of the SM22.alpha. promoter.
Claims18
A method of modulating smooth muscle cell proliferation in a mammal comprising the step of providing to a smooth muscle cell in said mammal an adenoviral construct comprising an SM22.alpha. promoter operably linked to a nucleotide sequence encoding a cell cycle regulatory molecule, said SM22.alpha. promoter having a maximum of 441 bases and comprising
- (a) bases 900-1340 of SEQ ID NO:1 or
- (b) a sequence hybridizing with the complement of SEQ ID NO:1 under hybridizing conditions comprising 0.02M-0.15 M sodium chloride at temperatures of 50.degree. C. to 70.degree. C.,
- such that said cell cycle regulatory molecule is expressed when said construct is provided to said smooth muscle cell.
A method of modulating smooth muscle cell proliferation in a mammal comprising the step of providing to a smooth muscle cell in said mammal an adenoviral construct comprising an SM22.alpha. promoter operably linked to a nucleotide sequence encoding a cell cycle regulatory molecule, said promoter having a maximum of 441 bases and comprising
- (a) an oligomer of at least one DNA sequence selected from the group consisting of SME1 (SEQ ID NO:21), SME2 (SEQ ID NO:23), SME3 (SEQ ID NO:25), SME4 (SEQ ID NO:27), SME5 (SEQ ID NO:31), and SME6 (SEQ ID NO:33), or
- (b) a sequence that hybridizes with the complement of at least one of the group consisting of:
- SME1 (SEQ ID NO:21), SME2 (SEQ ID NO:23), SME3 (SEQ ID NO:25), SME4 (SEQ ID NO:27), SME5 (SEQ ID NO:31), and SME6 (SEQ ID NO:33) under hybridizing conditions comprising 0.02M-0.15 M sodium chloride at temperatures of 50.degree. C. to 70.degree. C.,
- such that said nucleotide sequence encoding said cell cycle regulatory molecule is expressed when said construct is provided to said smooth muscle cell.
Dependencies are inferred from the claim text.