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AssigneeMedical Analysis Systems, Inc.
InventorsJozsef Hepp, Zsolt Lengyel, Rajiv Pande, Janos Botyanszki, Miklos Sahin-Toth
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The present invention relates to a method, kit and reagent composition for determining the presence of a target nucleic acid in a sample using a two-stage target cycling reaction. During stage 1 of the reaction, the target nucleic acid hybridizes with a probe nucleic acid to effectuate release of an activator into the assay medium. During stage 2 of the reaction, a target analog-anchor complex is cleaved by the activator, which accomplishes the dual purpose of releasing the target analog into the assay medium, and the initiation of signal generation. The released target analog then restarts the cyclic reaction by binding to a second probe, which effectuates release of a second activator, and so on. This cyclic reaction gives rise to an increase in the amount of signal generated from a single target nucleic acid molecule in the sample, which greatly enhances the level of target detection that can be expected.
A method for performing target amplification in an assay system comprising an assay medium, said assay system being useful for determining the presence of a target nucleic acid having a target nucleic acid sequence in a sample suspected of containing said target nucleic acid, comprising the steps of:
A method for increasing the sensitivity of an assay for detecting a target nucleic acid in a sample by releasing a target nucleic acid analog into an assay medium in the presence of the target nucleic acid, wherein the target nucleic analog generates a signal similarly with that of the target nucleic acid, thereby amplifying the amount of nucleic acid which is detected in the assay, comprising the steps of:
Dependencies are inferred from the claim text.