# US 6,114,110 A · Isolation and propagation of a human herpesvirus derived from AIDS-associated Kaposi's sarcoma cells

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 6,114,110 A
- Document ID: US6114110A
- Status: Granted
- Kind: A
- Application number: 08/585,588
- Filed: 1996-01-16
- Granted: 2000-09-05
- Earliest claimed: 1996-01-16
- Assignees: University of Michigan
- Inventors: Gary J. Nabel; Brian J. Nickoloff
- Web page: https://patentgrep.com/patent/US6114110A

## CPC

- G01N 33/5091: {for testing the pathological state of an organism}
- A61K 39/12: Viral antigens
- A61K 39/245: Herpetoviridae, e.g. herpes simplex virus
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics}
- G01N 33/5011: {for testing antineoplastic activity}
- G01N 33/5064: {Endothelial cells}
- G01N 33/56994: {Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus}
- C12N 2710/16421: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2710/16434: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

This present invention demonstrates the presence of a replication-competent herpesvirus associated with AIDS-associated Kaposi's sarcoma cells, vaccines derived therefrom, methods for diagnosing for Kaposi sarcoma, and methods for screening for antiviral drugs effective against human herpesvirus.

## Claims

### Claim 1

A method of cultivating KS cells according to the present invention comprises the steps of:
isolating a KS cell from a KS lesion or from tissue taken from a patient biopsy; and
culturing said KS cell on a medium coated with endothelial cell attachment factor.

### Claim 2 (depends on 1)

The method of claim 1, wherein said KS cell is taken from a KS lesion derived from a patient with AIDS.

### Claim 3 (depends on 1)

The method of claim 1, wherein said medium is RPMI medium supplemented with antibiotics, serum and growth factors.

### Claim 4

A method for propagating a cell infected with human herpesvirus 8 (HHV-8) comprising:
contacting a culture of epithelial cells or umbilical endothelial cord cells with a cell or cell lysate of a HHV-8 to form an infected cell culture,
incubating the infected cell culture for at least 1 day, and
passaging the infected cell culture.

### Claim 5 (depends on 4)

The method of claim 4, wherein said epithelial cell is a embryonic epithelial cell.

### Claim 6 (depends on 5)

The method of claim 5, wherein said embryonic epithelial cell is a human embryonic kidney epithelial cell.

### Claim 7 (depends on 5)

The method of claim 5, wherein said human embryonic kidney epithelial cell is a 293 cell.

### Claim 8 (depends on 4)

The method of claim 4, wherein the infected cell culture is incubated for at least 3 days prior to passaging.

### Claim 9

A method of detecting the presence of human herpesvirus 8 (HHV-8) in a biological fluid comprising:
contacting said biological fluid from said patient with an antibody specific HHV-8, and
detecting the presence of complexes of antibody and human herpesvirus.

### Claim 10 (depends on 9)

The method of claim 9, wherein said biological fluid is blood, semen or urine.

### Claim 11

A method of detecting the presence of antibodies selective for human herpesvirus 8 (HHV-8) in a biological fluid comprising:
contacting said biological fluid from said patient with HHV-8 viral antigen so as to form complexes between said viral antigen and any antibodies selective for HHV-8,
detecting the presence of said complexes of antibody and viral antigen.

### Claim 12

A method of screening for antiviral activity against human herpesvirus 8 (HHV-8), comprising:
cultivating a cell line infected with HHV-8 for at least 1 day,
contacting said infected cell culture with an antiviral drug candidate to form an exposed cell culture,
culturing the exposed cell in the presence of the antiviral drug candidate, and
determining the effect of the antiviral drug candidate.