Self-assembling polynucleotide delivery system comprising dendrimer polycations
AssigneeThe Regents of the University of California
InventorsFrancis C. Szoka, Jr., Jean Haensler
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Abstract
A self-assembling polynucleotide delivery system comprises a dendrimer polycation aiding in the delivery of the polynucleotide to a desired address, and optionally other agents such as DNA masking agents, cell recognition agents, charge-neutralization agents, membrane-permeabilization agents, and subcellular-localization agents.
Claims64
A composition for presenting a polynucleotide to a eukaryotic cell, comprising
- a polynucleotide;
- and
- a dendrimer polycation having terminal cationic groups non-covalently coupled to the polynucleotide;
- wherein the proportion of terminal cationic groups of the dendrimer polycation to nucleotide content of the polynucleotide is 1:1 to 223.3:1.
The composition of clainm 5, wherein the dendrimer polycation further comprises 0 to about 90% terminal reactive residues of all terminal groups of the dendrimer polycation.
The composition of 19 wherein the .beta.-pleated sheet amphipathic peptide has a first and second face such that the first face is positively charged and the second face is substantially neutral.
Dependencies are inferred from the claim text.