LICC of Streptococcus pneumoniae
AssigneeSmithKline Beecham Corporation
InventorMichael Arthur Lonetto
Life of this patent
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Abstract
The invention provides licC polypeptides and polynucleotides encoding licC polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing licC polypeptides to screen for antibacterial compounds.
Claims34
An isolated polynucleotide comprising a first polynucleotide or the complement of the entire length of said first polynucleotide, wherein said first polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2.
An isolated polynucleotide comprising a first polynucleotide or the complement of the entire length of said first polynucleotide, wherein said first polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 4.
An isolated polynucleotide comprising a first polynucleotide or the complement of the entire length of said first polynucleotide, wherein the first polynucleotide encodes a polypeptide comprising an amino acid sequence identical to SEQ ID NO:2 except that, over the entire length corresponding to SFEQ ED NO:2, the amino acid sequence has a substitution, deletion or insertion of one amino acid.
An isolated polynucleotide comprising a first polynucleotide or the complement of the entire length of said first polynucleotide, wherein said first polynucleotide encodes a polypeptide comprising an amino acid sequence identical to SEQ ID NO:
- 4 except that, over the entire length corresponding to SEQ ID NO:4, the amino acid sequence has a substitution, deletion or insertion of one amino acid.
An isolated polynucleotide comprising a first polynucleotide or the full complement of the entire length of the first polynucleotide, wherein the first polynucleotide is selected from the group consisting of:
- (a) a polynucleotide consisting of nucleotides 1 to 687 set forth in SEQ ID NO:1;
- (b) a polynucleotide consisting of the nucleotide sequence set forth in SEQ ID NO:3;
- and,
- (c) a polynucleotide identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), n.sub.a nucleotides are substituted, inserted or deleted, wherein n.sub.a satisfies the following expression
- n.sub.a .ltoreq.x.sub.a -(x.sub.a .cndot.y)
- wherein x.sub.a is the total number of nucleotides in the polynucleotide of (a), y is 0.99, and wherein any non-integer product of x.sub.a and y is rounded down to the nearest integer before subtracting the product from x.sub.a.
An isolated polynucleotide comprising a first polynucleotide or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes the same mature licC polypeptide as expressed by a polynucleotide comprising SEQ ID NO:1 or SEQ ID NO:
- 3 contained in NCIMB Deposit No. 40794.
Dependencies are inferred from the claim text.