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US 6,107,543 AGranted
Culture of totipotent embryonic inner cells mass cells and production of bovine animals
AssigneeInfigen, Inc.
InventorsMichelle M. Sims, Neal L. First
Life of this patent
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Abstract
The use of totipotent embryonic stem cells to provide substantially identical cells for embryo cloning techniques is described. The method includes the culture of loose suspensions of inner cell mass cells of bovine animals to retrieve large populations of stem cells. The invention also describes the use of stem cells in various genetic manipulation techniques.
Claims6
A method for producing a bovine animal, the method comprising:
- (a) providing a cultured bovine embryonic inner cell mass cell, wherein said inner cell mass cell has been cultured in a culture medium for a length of time sufficient for maintenance of said inner cell mass cell in an undifferentiated state;
- (b) establishing an embryo from a nuclear transfer process, wherein said nuclear transfer process comprises the step of inserting said cultured bovine embryonic inner cell mass cell into an enucleated bovine oocyte, and the step of activating said embryo;
- (c) implanting said embryo into a recipient bovine host;
- and
- (d) allowing said embryo to develop into the bovine animal.
2 more elements
Dependencies are inferred from the claim text.