High amylose starch from transgenic potato plants
AssigneeNational Starch and Chemical Investment Holding Corporation
InventorsDavid Cooke, Michael John Gidley, Stephen Alan Jobling, Richard Safford, Christopher Michael Sidebottom, Roger J. Westcott
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Abstract
Disclosed is a method of producing altered starch from transformed potato plants or their progeny, comprising extracting starch from a potato plant, at least the tubers of which comprise at least an effective portion of a starch branching enzyme (SBE) cDNA sequence operably linked in the antisense orientation to a suitable promoter, such that the level of SBE activity is limited to less than 0.8 units per gram tuber. Also disclosed are potato plants comprising altered starch in accordance with the invention.
Claims19
A method of producing altered starch from transformed potato plants or their progeny, the method comprising extracting starch from a potato plant, at least the tubers of which comprise at least an effective portion of a starch branching enzyme (SBE) cDNA sequence operably linked in the antisense orientation to a suitable promoter, such that the level of SBE activity is limited to less than 0.8 units per gram tuber.
An altered starch, wherein the starch is extracted from transformed potato plants or their progeny, the plants having less than 0.8 units SBE activity per gram tuber, and, as extracted, the starch has the following physical properties:
- a) elevated peak temperature of gelatinisation as determined by differential scanning calorimetry (DSC) relative to unaltered starch extracted from equivalent non-transformed plants;
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- b) elevated viscosity onset temperature, relative to unaltered starch extracted from equivalent non-transformed plants.
Dependencies are inferred from the claim text.