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AssigneesBattelle Memorial Institute, Medical University of South Carolina
InventorsYuehuei H. An, Vladimir A. Mironov, Anna Gutowska
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A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.
A gelling cell culture medium, useful for forming a three dimensional matrix for cell culture in vitro, the gelling cell culture medium, comprising:
A method of making a gelling cell culture medium for in vitro cell culturing, comprising the steps of:
A method of in-vitro propagation of phenotypical cells, comprising the steps of:
Dependencies are inferred from the claim text.