Spodoptera frugiperda single cell suspension cell line in serum-free media, methods of producing and using

An insect cell line established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 designated ATCC CRL-1771, through dilution and selection in a serum-free insect medium supplemented with added human insulin, which grows in a serum-free insect medium supplemented with added human insulin, and which is genetically and morphologically distinct from the parent Sf9 cells.

An insect cell line established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9, designated ATCC CRL-1771, through dilution and selection in a serum-free insect medium supplemented with added human insulin, which grows in a serum-free insect medium supplemented with added human insulin, and which is genetically and morphologically distinct from the parent Sf9 cells, and, which grow as essentially a suspension of single cells, and which can be passed continuously for at least 6 months while maintaining a level of viability of >98%.

A method for obtaining an insect cell line comprising subjecting Lepidoptera, Noctuidae, Spodoptera frugiperda Sf9 cells designated ATCC CRL-1771 to dilution and selection in a serum-free insect medium supplemented with added human insulin.