Enzyme and microorganism degrading rhamnogalacturonane II
AssigneeInstitute National de la Recherche Agronomique
InventorsPatrice Pellerin, Jean-Marc Brillouet, Thierry Doco, Pascale Williams, Stephane Vidal, Michel Moutounet
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Abstract
Enzyme degrading rhamnogalacturonane II (RG-II) having an endo-.beta.-L-rhamnopyranosyl-(I.fwdarw.3')-D-apiofuranosyl hydrolase activity and/or an endo-.alpha.-L-fucopyranosyl-(I.fwdarw.4)-L-rhamnopyranosyl hydrolase activity. This enzyme can be produced by a microorganism, especially of the penicillium species, such as the branches 1-1577 and 1-1578 lodged with the CNCM. The RG-II can obtained from plant extracts by means of a method comprising a chromatography stage.
Claims6
A total enzymatical extract having at least one activity selected from the group consisting of endo-.beta.-L-rhamnopyranosyl-(1-3)-D-apiofuranosylhydrolase activity and endo-L-1-fucopyranosyl- (1-4) -L-rhamnopyranosylhydrolase activity for degrading rhamnogalacturonane(RG-II) and its derivatives wherein said isolated enzyme is produced by a penicillium strain selected from the group consisting of strain No. I-1577 (IPV) and strain I-1578 (LAV2) deposited at the National Collection of Microorganism Cultures at Institut Pasteur.
Dependencies are inferred from the claim text.