Method for reducing phosphorus content of edible oils
AssigneeNovo Nordisk A/S
InventorsIb Groth Clausen, Shamkant Anant Patkar, Kim Borch, Martin Barfoed, Kim Clausen, Claus Crone Fuglsang, Lone Dybdal, Torben Halkier
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Abstract
The present invention relates to a method for reducing the content of phosphorous containing components in an edible oil comprising a high amount of non-hydratable phosphorous content, wherein the method comprises use of a phospholipase. Further the present invention relates to an enzyme with phospholipase activity, a cloned DNA sequence encoding the enzyme with phospholipase activity, a method of producing the enzyme, and the use of enzyme for a number of industrial applications.
Claims4
An isolated polypeptide selected from the group consisting of:
- (a) polypeptide exhibiting phospholipase A activity encoded by the DNA sequence cloned into plasmid pYES 2.0 present in Escherichia coli DSM 11299;
- (b) a polypeptide exhibiting phospholipase A activity having an amino acid sequence as shown in positions 31-346 of SEQ ID NO 2;
- (c) a polypeptide exhibiting phospholipase A activity having an amino acid sequence as shown in position 31-303 of SEQ ID NO 2;
- and (d) a polypeptide exhibiting phospholipase A activity which is at least 70% homologous with said polypeptide defined in (a), (b) or (c).
Dependencies are inferred from the claim text.