Chimeric Gag pseudovirions
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsGregory J. Tobin, Matthew A. Gonda
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Abstract
The present invention provides, inter alia, recombinant chimeric nucleic acids encoding a Gag-fs-fusion partner fusion protein; a pseudovirion comprising a retroviral Gag protein and a fusion partner, wherein the fusion partner is present in a Gag-fs-fusion partner fusion protein; an immunogenic composition comprising a pseudovirion; a Gag-fs-fusion partner fusion protein; and a method of making the pseudovirions of the present invention.
Claims43
A recombinant chimeric nucleic acid, comprising:
- a retroviral gag sequence;
- a target nucleic acid sequence derived from a nucleic acid encoding a fusion partner selected from the group consisting of Env, an interleukin, TNF, GM/CSF, a nonretroviral viral antigen and a cancer antigen;
- wherein the gag and target sequences are transcribed from a single start site of transcription, and wherein the gag and target sequences are in different reading frames;
- and,
- a frame-shift site.
A recombinant chimeric gag-env nucleic acid, comprising:
- a retroviral gag sequence;
- a retroviral env sequence;
- wherein the gag and env sequences are transcribed from a single start site of transcription, and wherein the gag and env sequences are in different reading frames;
- and,
- a retroviral frame-shift site derived from a retroviral gag-pol frame shift site.
A pseudovirion comprising a retroviral Gag protein and a fusion partner, wherein the fusion partner is present in a Gag-fs-fusion partner fusion protein.
An immunogenic composition comprising a pseudovirion comprising a retroviral Gag protein and a retroviral fusion partner, wherein the fusion partner is present in a Gag-fs-fusion partner fusion protein and wherein the fusion partner is derived from a retroviral Env protein.
A particulate vaccine comprising a pseudovirion comprising a retroviral Gag protein and a retroviral fusion partner, wherein the fusion partner is present in a Gag-fs-fusion partner fusion protein and wherein the fusion partner is derived from a retroviral Env protein.
A fusion protein comprising a retroviral Gag sequence, a translation reading frame switching sequence and a fusion partner.
A method of making a pseudovirion comprising expressing a nucleic acid encoding a Gag-fs-fusion partner fusion protein in a cell, wherein the cell translates the nucleic acid into a first protein comprising a Gag sequence, and a second protein comprising a gag sequence and a fusogenic partner.
A pseudovirion comprising a retroviral Gag protein and a fusion partner, wherein the fusion partner is present in Gag-fs-fusion partner fusion protein and wherein the fusion partner is derived from retroviral Env protein.
A fusion protein comprising a retroviral Gag sequence, a translation reading frame switching sequence and a retroviral Env amino acid subsequence.
Dependencies are inferred from the claim text.