Back to results
US 6,096,870 AGranted
Sequential separation of whey
AssigneeSepragen Corporation
InventorsZahid Mozaffar, Salah H. Ahmed, Vinit Saxena, Quirinus Ronnie Miranda
Life of this patent
Estimating the term…
Abstract
The present invention is related to the separation of whey proteins, particularly the sequential separation of whey proteins into separate fractions through the use of chromatography. The present invention further provides methods and compositions for the sequential separation of whey proteins, as well as their use in various products. The present invention also provides methods and compositions for the cleaning of chromatography resins used in the separation of whey proteins.
Claims66
A method for the sequential separation of whey proteins, comprising the steps of:
- a) providing:
- i) a cationic exchange resin contained within a container;
- ii) a whey sample, wherein said whey sample comprises at least one whey protein selected from the group consisting of immunoglobulin, .beta.-lactoglobulin, .alpha.-lactalbumin, lactoperoxidase, serum albumin, and lactoferrin;
- and
- iii) a cationic exchange resin;
- b) passing said whey sample through said resin under conditions whereby said whey proteins adsorb to said resin;
- c) collecting the flow-through from said cationic exchange resin, wherein said flow-through comprises lactose, minerals, lactic acid, and non-nitrogenous components;
- d) sequentially eluting said immunoglobulin and said .beta.-lactoglobulin from said cationic exchange resin;
- e) eluting said .alpha.-lactalbumin from cationic exchange resin;
- f) eluting said serum albumin from cationic exchange resin;
- g) eluting said lactoferrin from said cationic exchange resin;
- and
- h) eluting said lactoperoxidase from said cationic exchange resin;
- wherein a sequential separation of whey proteins is achieved.
11 more elements
A two-column method for the separation of whey proteins comprising the steps of:
- a) providing:
- i) anionic exchange resin present within a first container to produce a first resin;
- ii) a whey sample containing .beta.-lactoglobulin, immunoglobulins, .alpha.-lactalbumin, serum albumin, and lactoferrin, and a soluble portion;
- iii) cationic exchange resin present within a second container to produce a second resin;
- and
- iv) an ultrafiltration membrane;
- b) passing said whey sample through said first resin under conditions wherein said .beta.-lactoglobulin adsorbs to said first resin and said soluble portion passes through the column to form a flow-through;
- c) collecting said flow-through from said first resin, wherein said flow-through comprises .alpha.-lactalbumin, immunoglobulins, serum albumin and lactoferrin suitable for further processing;
- d) eluting said adsorbed .beta.-lactoglobulin from said first resin to produce an eluate;
- e) passing said flow-through collected in step c) through said ultrafiltration membrane to produce an ultrafiltrate;
- f) passing said ultrafiltrate through said second resin, under conditions such that immunoglobulins, .alpha.-lactalbumin, serum albumin, and lactoferrin adsorb to said second resin;
- g) eluting said immunoglobulins from said second resin;
- h) eluting said .alpha.-lactalbumin from said second resin;
- i) eluting said serum albumin from said second resin;
- and
- j) eluting said lactoferrin from said second resin;
- thereby separating whey proteins.
14 more elements
A method for processing whey comprising the steps of:
- a) providing:
- i) a cation exchange resin;
- and
- ii[i]) a whey sample, wherein said whey sample comprises whey proteins;
- b) passing said whey sample through said cation exchange resin under conditions whereby said whey proteins adsorb to said cation exchange resin, and a flow through passes through said cation exchange resin;
- c) collecting said flow-through, wherein said flow-through comprises deproteinized whey;
- thereby processing whey.
4 more elements
A method for the production of clear whey protein isolate comprising:
- a) providing:
- i) a cation exchange resin;
- and
- ii) a whey sample, wherein said whey sample comprises serum albumin, lipoprotein, whey protein, and a soluble portion;
- b) passing said whey sample through said cation exchange resin, under conditions that said whey protein, serum albumin, and lipoprotein adsorbs to said cation exchange resin, and said soluble portion flows through said cation exchange resin to produce a flow-through;
- c) eluting said whey protein adsorbed to said cation exchange resin to provide clear whey protein isolate;
- and
- d) eluting said serum albumin and fat from said cation exchange resin;
- thereby producing clear whey protein isolate.
6 more elements
A method for the production of .alpha.-lactalbumin-enriched whey protein isolate comprising:
- a) providing:
- i) anion exchange resin;
- and ii) whey comprising .alpha.-lactalbumin and .beta.-lactoglobulin;
- and
- b) passing said whey through said anion exchange resin under conditions such that said .beta.-lactoglobulin binds to said anion exchange resin and said .alpha.-lactalbumin flows through said anion exchange resin, to produce whey enriched in .alpha.-lactalbumin.
2 more elements
A method for cleaning resin contained within a chromatography column, comprising the steps of:
- a) providing:
- i) resin contained within a container;
- ii) sodium hypochlorite;
- iii) sodium hydroxide;
- iv) hydrochloric acid;
- v) ethanol;
- b) washing said resin with said sodium hypochlorite to produce washed resin;
- c) exposing said washed resin to said sodium hydroxide to produce a base-treated resin;
- d) exposing said base-treated resin to said hydrochloric acid to produce an acid-treated resin;
- and
- e) exposing said acid-treated resin with said ethanol to produce a cleaned resin;
- thereby cleaning the resin.
9 more elements
Dependencies are inferred from the claim text.