Methods for recovering polypeptides from plants and portions thereof
AssigneeBoard of Trustees, Rutgers, the State University of New Jersey
InventorIlya Raskin
Life of this patent
Estimating the term…
Abstract
Methods for using plants or intact portions thereof to produce and exude heterologous polypeptides are disclosed. The methods of the invention use whole plants or portions thereof that are intact, living and capable of exuding the produced polypeptides. The methods of the invention minimize operator intervention and exploit solar energy and the minimal nutrient needs of photoautotrophic organisms to provide inexpensive and indefinitely sustainable methods for producing a variety of heterologous polypeptides.
Claims11
In a process for recovery of a heterologous polypeptide from a plant having cells genetically transformed to express and secrete a heterologous polypeptide, the improvement comprising:
- (a) externally contacting said plant, or an intact portion thereof wherein expression occurs, with an aqueous medium to effect an admixture of said medium and an exudate of said plant or said intact portion thereof;
- and
- (b) recovering said polypeptide from said admixture.
In a process for recovery of a heterologous polypeptide from a plant having cells genetically transformed to express and secrete a heterologous polypeptide, the improvement comprising recovering said polypeptide from an exudate of said plant.
In a process for recovery of a heterologous polypeptide from a plant having cells containing a coding region for expression and secretion of a heterologous polypeptide, the improvement comprising:
- (a) externally contacting said plant, or an intact portion thereof wherein expression occurs, with a medium to effect an admixture of said medium and an exudate of said plant or said intact portion thereof;
- and
- (b) recovering said polypeptide from said admixture.
Dependencies are inferred from the claim text.