Human calcium channel compositions and methods
AssigneeSibia Neurosciences, Inc.
InventorsMichael M. Harpold, Steven B. Ellis, Mark E. Williams, Ann F. McCue
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Abstract
Isolated DNA encoding each of human calcium channel .alpha..sub.1 -, .alpha..sub.2 -, .beta.- and .gamma.-subunits, including subunits that arise as splice variants of primary transcripts, is provided. Cells and vectors containing the DNA and methods for identifying compounds that modulate the activity of human calcium channels are also provided.
Claims29
An isolated DNA molecule, comprising a sequence of nucleotides that encodes a .beta..sub.3-1 -subunit of a human calcium channel, wherein:
- the sequence of nucleotides encoding the .beta..sub.3-1 -subunit of a human calcium channel is selected from the group consisting of:
- (a) a sequence of nucleotides that encodes a .beta..sub.3-1 -subunit of a human calcium channel and that comprises the sequence of nucleotides set forth in SEQ ID No. 19;
- (b) a sequence of nucleotides that encodes a .beta..sub.3-1 -subunit of a human calcium channel, wherein:
- the sequence of nucleotides hybridizes along its full length under conditions of high stringency to the complement of the sequence of nucleotides set forth in SEQ ID no.19 including the portion of SEQ ID No. 19 that encodes amino acids 1-15;
- and the sequence of nucleotides is fully complementary to an mRNA transcript native to a human cell;
- (c) a sequence of nucleotides that encodes a .beta..sub.3-1 -subunit of a human calcium channel that comprises the sequence of amino acids encoded by the sequence set forth in SEQ ID Nos. 19;
- and
- (d) a sequence of nucleotides degenerate with the human calcium channel .beta..sub.3-1 -subunit-encoding sequence of (b).
An isolated DNA molecule comprising a sequence of nucleotides that encodes a .beta..sub.3-1 -subunit of a human calcium channel that is encoded by the sequence of nucleotides set forth in SEQ ID No. 19.
An isolated recombinant DNA molecule, comprising nucleotides 122-457 in SEQ ID No. 19.
A plasmid having all of the identifying characteristics of the plasmid harbored in the Escherichia coli (E. coli) host cells having ATCC Accession No. 69048.
A eukaryotic cell, comprising heterologous nucleic acid that encodes a .beta..sub.3-1 -subunit of a human calcium channel encoded by a sequence of nucleotides selected from the group consisting of:
- (a) a sequence of nucleotides that encodes a human .beta..sub.3-1 -subunit and that comprises the sequence of nucleotides set forth in SEQ ID No. 19;
- (b) a sequence of nucleotides that encodes a full-length .beta..sub.3-1 -subunit, wherein:
- the sequence of nucleotides hybridizes along its full length under conditions of high stringency to the complement of the sequence of nucleotides set forth in SEQ ID no.19 including the portion of SEQ ID No. 19 that encodes amino acids 1-15;
- and
- the sequence of nucleotides is fully complementary to an mRNA transcript native to a human cell;
- (c) a sequence of nucleotides that encodes a .beta..sub.3-1 -subunit that comprises the sequence of amino acids encoded by the sequence set forth in SEQ ID Nos. 19;
- and
- (d) a sequence of nucleotides degenerate with the .beta..sub.3-1 -subunit-encoding sequence of (b).
Dependencies are inferred from the claim text.