Compositions containing thrombin and microfibrillar nanometer collagen, and methods for preparation and use thereof
AssigneeCohesion Technologies, Inc.
InventorsJeffrey J. Prior, Donald G. Wallace, David H. Sierra, Frank A. DeLustro
Life of this patent
Estimating the term…
Abstract
The present invention relates to thrombin-containing hemostatic compositions, their preparation and use. In particular, it relates to hemostatic compositions comprising stabilized thrombin and microfibrillar collagen in an aqueous medium. In a preferred embodiment of the present invention, the compositions are used in a kit comprising two different components, one of which is autologous patient's plasma as the source of fibrinogen, and the other of which is the thrombin-containing composition which also contains microfibrillar collagen having an average diameter of about 3-30 nanometers.
Claims66
A hemostatic composition comprising thrombin and a mixture of non-microfibrillar collagen and microfibrillar collagen in an aqueous medium wherein said microfibrillar collagen has an average fibril diameter of about 3-30 nm.
A method for promoting hemostasis of a tissue site on or in a body of a mammalian subject comprising the steps of:
- (a) providing a hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium wherein said microfibrillar collagen has an average fibril diameter of about 3-30 nm;
- (b) providing an aqueous medium comprising fibrinogen;
- (c) mixing the hemostatic composition and the aqueous medium comprising fibrinogen immediately prior to contact with the tissue site;
- and
- (d) contacting the mixture with the tissue site.
A method for promoting hemostasis of a tissue site on or in a body of a mammalian subject comprising the steps of:
- (a) providing a hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium wherein said microfibrillar collagen has an average fibril diameter of about 3-30 nm;
- (b) providing an aqueous medium comprising platelets;
- (c) mixing the hemostatic composition and the aqueous medium comprising platelets immediately prior to contact with the tissue site;
- and
- (d) contacting the mixture with the tissue site.
A method of making a composition for use as a hemostatic agent comprising the steps of:
- (a) mixing thrombin, fibrillar collagen and a fiber disassembly agent together in an aqueous medium, wherein the fiber disassembly agent is present at a concentration which is effective at disassembling the fibrillar collagen into microfibrillar collagen;
- and
- (b) incubating the mixture formed in step (a) for a sufficient length of time and at a sufficient temperature to form microfibrillar collagen having a stable fiber size wherein said microfibrillar collagen has an average fibril diameter of about 3-30 nm.
A hemostatic composition comprising thrombin, polyethylene glycol and microfibrillar collagen in an aqueous medium wherein said microfibrillar collagen has an average fibril diameter of about 3-30 nm.
Dependencies are inferred from the claim text.