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US 6,096,304 AGranted
Recombinant baculovirus insecticides
AssigneeE. I. Du Pont de Nemours and Company
InventorBilly Fred McCutchen
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AssigneeE. I. Du Pont de Nemours and Company
InventorBilly Fred McCutchen
Estimating the term…
This invention pertains to recombinant baculoviruses that have been engineered to afford optimal expression of genes encoding insect-selective neurotoxins. More specifically, this invention pertains to an isolated nucleic acid sequence encoding the scorpion toxin LqhIT2, derived from Leiurus quinquestriatus hebraeus, wherein the sequence has been optimized for gene expression in nuclear polyhedrosis virus-infected cells. This invention also pertains to chimeric genes comprising a codon-optimized LqhIT2 nucleotide sequence, insecticidal compositions comprising recombinant baculoviruses expressing a codon-optimized, insect-selective neurotoxin (e.g. the LqhIT2 toxin gene), and methods for controlling insects in both agronomic and non-agronomic environments comprising application of insect baculoviruses containing the codon-optimized nucleic acid sequence encoding an insect-selective neurotoxin such as the LqhIT2 toxin.
A synthetic gene encoding an insect-selective neurotoxin comprising a coding nucleotide sequence that has been optimized for gene expression based on codon bias of baculoviruses or of cells in which baculoviruses replicate wherein said synthetic gene comprises the nucleotide sequence of SEQ ID NO:12.
Dependencies are inferred from the claim text.