# US 6,093,700 A · Method of inducing an immune response using vaccinia virus recombinants encoding GM-CSF

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- Number: US 6,093,700 A
- Document ID: US6093700A
- Status: Granted
- Kind: A
- Application number: 08/732,505
- Filed: 1997-02-20
- Granted: 2000-07-25
- Earliest claimed: 1997-02-20
- Assignees: Thomas Jefferson University
- Inventors: Michael J. Mastrangelo; Edmund C. Lattime; David Berd; Laurence C. Eisenlohr
- Web page: https://patentgrep.com/patent/US6093700A

## CPC

- C12N 15/86: Viral vectors
- A61K 38/193: {Colony stimulating factors [CSF]}
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C12N 2710/24143: viral genome or elements thereof as genetic vector
- C12N 2710/24171: Demonstrated in vivo effect
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A method of inducing expression of immune active cytokines in tumors in situ is provided wherein a vaccinia virus vector capable of inducing expression of a selected cytokine is generated and injected into a tumor so that cells of the tumor express the selected cytokine. A method of enhancing immunity in a host by administration of a vaccinia virus vector is also provided. Methods of treating cancer by administration of these vaccinia virus vectors are also provided.

## Claims

### Claim 1

A method of expressing granulocyte-macrophage colony stimulating factor in tumors in situ comprising: a) generating a vaccinia virus vector encoding a gene for granulocyte-macrophage colony stimulating factor operably linked to transcriptional regulatory elements; and b) injecting said vaccinia virus into a tumor so that cells of the tumor express granulocyte-macrophage colony stimulating factor.

### Claim 2

A method of producing regression of a tumor in a mammal having cancer comprising administering to the tumor in said mammal a vaccinia virus vector encoding a gene for granulocyte-macrophage colony stimulating factor operably linked to transcriptional regulatory elements in an amount capable of inducing an immune response to the tumor in the mammal such that the tumor regresses.

### Claim 3 (depends on 2)

The method of claim 2 wherein the animal has cancer comprising bladder cancer, head cancer, neck cancer or melanoma.

### Claim 4 (depends on 2)

The method of claim 2 wherein the animal has prostate cancer.