Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP

An isolated and purified CryFT29 crystal protein comprising SEQ ID NO:2 having insecticidal activity against the southern corn rootworrn, Diabrotica undecimpunctata howardi, the Colorado potato beetle, Leptinotarsa decemlineata, the western corn rootworm, Diabrotica virgifera virgifera, the Japanese beetle, Popillia japonica, the red flour beetle, Tribolium castaneum, or the cat flea, Ctenocephalides felis.

A composition comprising a CryET29 crystal protein comprising SEQ ID NO:2, wherein the composition is substantially free of intact cells.

A method of treating flea infestation of an animal, comprising administering to said animal a therapeutically-effective amount of a pharmaceutically-acceptable CryET29 composition, wherein said composition is substantially free of intact cells and comprises an isolated and purified CryET29 crystal protein comprising SEQ ID NO:2.

A biologically pure culture of Bacillus thuringiensis cells that produces a CryET29 crystal protein comprising SEQ ID NO:2.

A composition comprising a CryET29 crystal protein comprising SEQ ID NO:2 prepared by a process comprising the steps of:

• a) culturing a Bacillus thuringiensis EG4096 or EG11494 cell under conditions effective to produce a CryET29 crystal protein;
• and
• b) obtaining said CryET29 crystal protein from said cell.

A method of preparing a CryET29 crystal protein comprising:

• a) culturing a Bacillus thuringiensis EG4096 or EG11494 cell under conditions effective to produce a CryET29 crystal protein;
• and
• b) obtaining said CryET29 crystal protein from said cell, wherein said CryET29 crystal protein comprises SEQ ID NO:2.