Rupestris stem pitting associated virus nucleic acids, proteins, and their uses
AssigneeCornell Research Foundation, Inc.
InventorsDennis Gonsalves, Baozhong Meng
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Abstract
The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide of a Rupestris stem pitting associated virus. The encoding DNA molecule, either alone in isolated form, in an expression system, a host cell, or a transgenic grape plant, is also disclosed. Other aspects of the present invention relate to a method of imparting Rupestris stem pitting associated virus resistance to grape plants by transforming them with the DNA molecule of the present invention, and a method of detecting the presence of a Rupestris stem pitting associated virus, such as RSPaV-1, in a sample.
Claims4
A method for detecting a Rupestris stem pitting associated virus in a sample, said method comprising:
- providing a primer that binds to Rupestris stem pitting associated virus nucleic acid molecule as a probe in a gene amplification detection procedure under conditions that permit gene amplification to occur;
- contacting the sample with the primer;
- and
- detecting a gene amplification product indicating that Rupestris stem pitting associated virus is present in the sample.
Dependencies are inferred from the claim text.