Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
AssigneeIrori
InventorsJohn Cargill, Robert W. Armstrong
Life of this patent
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Abstract
The present invention provides labeled synthetic libraries of random oligomers and methods and apparatus for generating labeled synthetic oligomer libraries. Each member of such a library is labeled with a unique identifier tag that specifies the structure or sequence of the oligomer. In a preferred embodiment of the present invention the identifier tag is a microchip that is pre-encoded or encodable with information that is related back to a detector when the identifier tag is pulsed with electromagnetic radiation.
Claims49
A labeled synthetic oligomer library comprising a plurality of different members each member comprising an oligomer linked to an identifier which is a microchip encoded with information identifying the structure of said oligomer.
A labeled synthetic oligomer library produced by synthesizing on each of a plurality of pre-encoded substrates, each of which has a unique identifier tag, which is a microchip encoded with information, a single oligomer structure obtained by a method comprising the steps of:
- a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of transformation events;
- c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
- d) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- and
- e) repeating steps a) through c) from at least one to about twenty times.
A labeled synthetic oligomer library produced by synthesizing on each of a plurality of encodable substrates each of which contains a microchip encoded with information, a single oligomer structure obtained by a method comprising the steps of:
- a) apportioning said encodable substrates among a plurality of reaction vessels;
- b) exposing said encodable substrates in each reaction vessel to one or a plurality of transformation events;
- c) adding identifier information to said encodable substrates;
- d) apportioning said encodable substrates among a plurality of reaction vessels;
- and
- d) repeating steps a) through c) from at least one to about twenty times.
A method of preparing a labelled synthetic oligomer library comprising a plurality of different members, each member comprising a pre-encoded substrate linked to a single oligomer structure and bearing a unique identifier tag which is a microchip encoded with information identifying said oligomer structure, said method comprising the steps of:
- a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of transformation events;
- c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
- d) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- and
- e) repeating steps a) through c) from at least one to about twenty times.
A method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising an encodable substrate linked to a single oligomer structure and having a unique identifier tag which is a microchip encoded with information identifying said oligomer structure, said method comprising the steps of:
- a) apportioning said encodable substrates among a plurality of reaction vessels;
- b) exposing said encodable substrates in each reaction vessels to one or a plurality of transformation events;
- c) adding first identifier information to said encodable substrates;
- d) apportioning said encodable substrates among a plurality of reaction vessels;
- and
- e) repeating steps a) through c) from at least one to about twenty times.
A labeled synthetic oligomer library produced by synthesizing on each of a plurality of pre-encoded substrates, each of which has a unique identifier tag which is a microchip encoded with information, a single oligomer structure obtained by a method comprising the steps of:
- a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of monomers;
- c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
- d) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- and
- d) repeating steps a) through c) from at least one to about twenty times.
A labeled synthetic oligomer library produced by synthesizing on each of a plurality of encodable substrates each of which has a unique identifier tag which is a microchip encodable with information, a single oligomer structure obtained by a method comprising the steps of:
- a) apportioning said encodable substrates among a plurality of reaction vessels;
- b) exposing said encodable substrates in each reaction vessel to one or a plurality of monomers;
- c) adding identifier information to said encodable substrates;
- d) apportioning said encodable substrates among a plurality of reaction vessels;
- and
- d) repeating steps a) through c) from at least one to about twenty times.
A method of preparing a labelled synthetic oligomer library comprising a plurality of different members, each member comprising a pre-encoded substrate linked to a single oligomer structure and bearing a unique identifier tag identifying said oligomer structure, said method comprising the steps of:
- a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of monomers;
- c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
- d) apportioning said pre-encoded substrates among a plurality of reaction vessels;
- and
- e) repeating steps a) through c) from at least one to about twenty times.
A method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising an encodable substrate linked to a single oligomer structure and having a unique identifier tag which is a microchip encoded with information identifying said oligomer structure, said method comprising the steps of:
- a) apportioning said encodable substrates among a plurality of reaction vessels;
- b) exposing said encodable substrates in each reaction vessels to one or a plurality of monomers;
- c) adding first identifier information to said encodable substrates;
- d) apportioning said encodable substrates among a plurality of reaction vessels;
- and
- e) repeating steps a) through c) from at least one to about twenty times.
Dependencies are inferred from the claim text.