Optical characterization of nucleic acids and oligonucleotides
OwnerKarl Otto Gruelich, Claus Seidel +5inventors
InventorsKarl Otto Gruelich, Claus Seidel, Juergen Wolfrum, Manfred Auer, Matthias Gautel, Roger Goody, Siegfried Labeit
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Abstract
A method for the enzymatic sequencing of DNA is disclosed which uses dideoxythio-nucleotides or dideoxyamino-nucleotides as terminators that are labeled with one or two fluorescent dyes coupled to the terminators either before or after polymerization. The dye labeled DNA fragments are separated in a single lane of a separation system and then the terminal bases of each of the fragments are identified by measuring the fluorescent lifetime of the dye attached to the terminators.
Claims5
A method for the enzymatic sequencing of DNA comprising
- (a) performing a template-controlled polymerization of deoxynucleotide triphosphates with a polymerase and a dideoxythio-nucleotide or a dideoxyamino-nucleotide as a terminator, to form a mixture of DNA fragments wherein each DNA fragment has a terminator bonded to one of its terminal bases which terminator;
- (b) reacting the mixture of DNA fragments with one or two fluorescent dyes to couple at least one of the dyes to the terminator of each DNA fragment of the mixture;
- (c) separating the dye labeled DNA fragments in one lane of a separation system, and
- (d) identifying the terminal bases of the DNA fragments to which the terminator has been coupled by measurement of the fluorescent lifetime of the fluorescent dye.
Dependencies are inferred from the claim text.