Herbicide-tolerant protoporphyrinogen oxidase ("protox") genes
AssigneeNovartis AG
InventorsSandra L. Volrath, Marie A. Johnson, Eric R. Ward, Peter B. Heifetz
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Abstract
The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme.
Claims51
A DNA molecule comprising a modified plant nucleotide sequence that encodes a modified enzyme having protoporphyrinogen oxidase (protox) activity, wherein said modified enzyme comprises at least one amino acid substitution relative to the corresponding wild-type enzyme, wherein said DNA molecule is capable of being incorporated into a nucleic acid construct used to transform a plant, such that upon transformation with said nucleic acid construct said plant is rendered herbicide-tolerant.
A DNA molecule comprising a modified plant nucleotide sequence that encodes a modified enzyme having protoporphyrinogen oxidase (protox) activity, wherein said modified enzyme is resistant to an inhibitor of the corresponding wild-type enzyme, wherein said modified enzyme comprises at least one amino acid sub-sequence selected from the group consisting of:
- (a) AP.DELTA..sub.1 F (SEQ ID NO:38), wherein .DELTA..sub.1 is an amino acid other than arginine;
- (b) F.DELTA..sub.2 S, wherein .DELTA..sub.2 is an amino acid other than cysteine;
- (c) Y.DELTA..sub.3 G, wherein .DELTA..sub.3 is an amino acid other than alanine;
- (d) A.DELTA..sub.4 D, wherein .DELTA..sub.4 is an amino acid other than glycine;
- (e) Y.DELTA..sub.5 P, wherein .DELTA..sub.5 is an amino acid other than proline;
- (f) P.DELTA..sub.6 A, wherein .DELTA..sub.6 is an amino acid other than valine;
- (g) .DELTA..sub.7 IG, wherein .DELTA..sub.7 is an amino acid other than tyrosine;
- (h) YIGG.DELTA..sub.8 (SEQ ID NO:39), wherein .DELTA..sub.8 is an amino acid other than alanine or serine;
- (i) A.DELTA..sub.9 P, wherein .DELTA..sub.9 is an amino acid other than isoleucine;
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- (j) G.DELTA..sub.10 A, wherein .DELTA..sub.10 is an amino acid other than valine.
A DNA molecule comprising a modified plant nucleotide sequence that encodes a modified enzyme having protoporphyrinogen oxidase (protox) activity, wherein said modified enzyme is resistant to an inhibitor of the corresponding wild-type enzyme, wherein said nucleotide sequence is further characterized in that at least one of the following conditions is met:
- (a) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence AP.DELTA..sub.1 F (SEQ ID NO:38), wherein .DELTA..sub.1 is an amino acid other than arginine;
- (b) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence F.DELTA..sub.2 S, wherein .DELTA..sub.2 is an amino acid other than cysteine;
- (c) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence Y.DELTA..sub.3 G, wherein .DELTA..sub.3 is an amino acid other than alanine;
- (d) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence A.DELTA..sub.4 D, wherein .DELTA..sub.4 is an amino acid other than glycine;
- (e) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence Y.DELTA..sub.5 P, wherein .DELTA..sub.5 is an amino acid other than proline;
- (f) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence P.DELTA..sub.6 A, wherein .DELTA..sub.6 is an amino acid other than valine;
- (g) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence .DELTA..sub.7 IG, wherein .DELTA..sub.7 is an amino acid other than tyrosine;
- (h) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence YIGG.DELTA..sub.8 (SEQ ID NO:39), wherein .DELTA..sub.8 is an amino acid other than alanine or serine;
- (i) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence A.DELTA..sub.9 P, wherein .DELTA..sub.9 is an amino acid other than isoleucine;
- (j) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence G.DELTA..sub.10 A, wherein .DELTA..sub.10 is an amino acid other than valine;
- (k) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence Y.DELTA..sub.3 G, wherein .DELTA..sub.3 is an amino acid other than alanine, and said modified plant nucleotide sequence also has a sequence that encodes one of the group consisting of:
- (1) sub-sequence Q.DELTA..sub.11 S, wherein .DELTA..sub.11 is an amino acid other than proline,
- (2) sub-sequence IGG.DELTA..sub.12 (SEQ ID NO:40), wherein .DELTA..sub.12 is an amino acid other than threonine,
- (3) sub-sequence SWXL.DELTA..sub.13 (SEQ ID NO:41), wherein .DELTA..sub.13 is an amino acid other than serine,
- (4) sub-sequence L.DELTA..sub.14 Y, wherein .DELTA..sub.14 is an amino acid other than asparagine, and
- (5) sub-sequence G.DELTA..sub.15 XGL (SEQ ID NO:42), wherein .DELTA..sub.15 is an amino acid other than tyrosine;
- (l) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence .DELTA..sub.7 IG, wherein .DELTA..sub.7 is an amino acid other than tyrosine, and said modified plant nucleotide sequence also has a sequence that encodes one of the group consisting of:
- (1) sub-sequence Q.DELTA..sub.11 S, wherein .DELTA..sub.11 is an amino acid other than proline,
- (2) sub-sequence IGG.DELTA..sub.12 (SEQ ID NO:40), wherein .DELTA..sub.12 is an amino acid other than threonine,
- (3) sub-sequence SWXL.DELTA..sub.13 (SEQ ID NO:41), wherein .DELTA..sub.13 is an amino acid other than serine,
- (4) sub-sequence L.DELTA..sub.14 Y, wherein .DELTA..sub.14 is an amino acid other than asparagine, and
- (5) sub-sequence G.DELTA..sub.15 XGL (SEQ ID NO:42), wherein .DELTA..sub.15 is an amino acid other than tyrosine;
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- (m) said modified plant nucleotide sequence has a sequence that encodes amino acid sub-sequence T.DELTA..sub.16 G, wherein .DELTA..sub.16 is an amino acid other than leucine, and said modified plant nucleotide sequence also has a sequence that encodes amino acid sub-sequence YV.DELTA..sub.17 G (SEQ ID NO:43), wherein .DELTA..sub.17 is an amino acid other than alanine.
Dependencies are inferred from the claim text.