US 6,083,733 AGranted

Thermostable xylanases

AssigneeGist-Brocades N.V.

InventorsVidar Gronberg, Simon Forster, Dean Moody, Diane P. Williams, Sara Iverson, Roberta Lee Farrell, Peter Leonard Bergquist, McIver Daniel, Hugh William Morgan, Wilhelmus Johannes Quax, Margareta Adriana Herweijer, Brian Edward Jones

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Abstract

The present invention discloses enzymes having xylanase activity. The xylanases are characterized in that they are active at a temperature of 80.degree. C. or higher. The enzymes are obtainable from anaerobic thermophilic bacteria. The enzymes are suited for use in paper and pulp production processes. The invention also describes cloning and expression of genes having xylanase activity obtained from the deposited strains.

Claims12

Dependent claims

A purified and isolated G-type xylanase enzyme from a thermophilic microorganism, wherein the G-type xylanase has the following characteristics:

(a) has significant delignifying activity at a temperature of at least 80.degree. C. and at a pH of 9.0 or above;
and
(b) the encoding nucleotide sequence therefor comprises an internal consensus fragment (ICF) which has more than 80% sequence identity with the G-type internal consensus fragment, positions 295 to 623, of SEQ ID NO:12, or which has an amino acid sequence at least 70% identical with SEQ ID NO:
13.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 08/591,685Filed 5 Feb 1997
Priority: EP 9420169914 Jun 1994
Granted: 4 Jul 2000
Term ends: Not estimatedThe source records don’t support an estimate.

Classification9 codes

D21C 5/005
Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting…:…
C12N 9/2482
Xylanases: endo-1,4-beta-xylanase
C12Y 302/01008
Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds: endo-1,4-beta-xylanase
D21C 9/1036
Other features in bleaching processes: use of compounds accelerating or improving the efficiency of the processes