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US 6,083,728 AGranted
Production of glutamate using wild type Bacillus methanolicus
AssigneeRegents of the University of Minnesota
InventorsFrederick J. Schendel, Richard Dillingham, Richard S. Hanson, Konosuke Sano, Kazuhiko Matsui
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Abstract
A method of producing glutamic acid by culturing a biologically pure wild type Bacillus methanolicus which exhibits sustained growth at 50.degree. C. using methanol as a carbon and energy source and requiring vitamin B.sub.12 and biotin is provided.
Claims23
A method for producing glutamic acid, the method comprising the steps of:
- culturing a biologically pure culture of a non-auxotrophic Bacillus methanolicus isolated from a natural source in nutrient medium comprising methanol as the primary carbon source, a copper ion, and biotin at a cell density of more than about 20 g/L cell dry weight at a temperature of about 45.degree. C. to about 60.degree. C. until glutamic acid is produced and excreted at a concentration of at least about 20 g/L;
- and
- recovering the excreted glutamic acid from the nutrient media.
A method for producing glutamic acid, the method comprising the steps of:
- culturing a biologically pure culture of a non-auxotrophic Bacillus methanolicus isolated from a natural source in nutrient medium comprising methanol as the primary carbon source, a copper ion, a surfactant, and a greater than limiting amount of biotin at a cell density of more than about 20 g/L cell dry weight at a temperature of about 45.degree. C. to about 60.degree. C. wherein glutamic acid is produced and excreted with a carbon conversion of methanol to glutamic acid of at least about 20%;
- and
- recovering the excreted glutamic acid from the nutrient media.
A method for producing glutamic acid, the method comprising the steps of:
- culturing a biologically pure culture of a non-auxotrophic Bacillus methanolicus isolated from a natural source in nutrient medium comprising methanol as the primary carbon source and a limiting amount of magnesium ion at a temperature of about 45.degree. C. to about 60.degree. C. until glutamic acid is produced and excreted at a concentration of at least about 20 g/L;
- and
- recovering the excreted glutamic acid from the nutrient media.
Dependencies are inferred from the claim text.