Rapid assays for the assessment of organ status based on the detection of one or more isoenzymes of glutathione S-transferase
AssigneeBiotrin Intellectual Properties Limited
InventorsJohn Martin Doyle, Cormac Gerard Kilty
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Abstract
The present invention is drawn to a method for the rapid assessment of organ status, including organ damage following immunological or toxicological insult, in a subject based on the detection of one or more isoenzymes of glutathione S-transferase (GST) in diverse biological fluids comprising contacting a particle-labelled anti-GST antibody specific for said isoenzyme with a sample of a biological fluid suspected of containing said isoenzyme, said antibody having a sensitivity sufficient to detect at least a picomolar amount of said isoenzyme, and capturing the particle-labelled antibody-isoenzyme complex on an immobolised capture antibody to generate a visually detectable signal.
Claims14
A method for the rapid assessment of organ status in a subject based on the detection of one or more isoenzymes of gluthatione S-transferase (GST) in biological fluids, which method comprises contacting a particle-labelled anti-GST antibody specific for said isoenzyme with a sample of a biological fluid suspected of containing said isoenzyme, said antibody having a sensitivity sufficient to detect at least a picomolar amount of said isoenzyme, and capturing the particle-labelled antibody-isoenzyme complex on an immobilised capture antibody to generate a visually detectable signal, wherein a presence or absence of said isoenzyme, as detected by a visual signal generated without an amplification step, indicates organ status.
Dependencies are inferred from the claim text.