Shuttle vectors for the introduction of DNA into mycobacteria and utilization of such bacteria as vaccines
AssigneesInstitute National de la Sante et de la Recherche Medical (Inserm), Institut Pasteur de Lille
InventorsVincent Escuyer, Alain Baulard, Patrick Berche, Camille Locht, Nadia Haddad
Life of this patent
Estimating the term…
Abstract
Shuttle vectors for inserting DNA in mycobacteria comprising at least one origin of functional replication in said mycobacteria, another origin of functional replication in other bacteria, an enzyme cutting site allowing the insertion of DNA coding for a protein capable of being expressed in the mycobacteria, characterized in that they also carry a gene providing on said mycobacteria resistance to a compound containing a heavy metal.
Claims7
A recombinant pathogenic or attenuated mycobacterium transformed with nucleic acid, wherein the transformation imparts to the mycobacterium resistance to a compound containing a heavy metal.
A mycobacterium which carries a vector, the vector comprising at least one origin of functional replication in said mycobacteria, another origin of functional replication in other bacteria, an enzyme cutting site allowing insertion of DNA coding for a protein capable of being expressed in the mycobacteria, wherein the vector also carries a gene providing on said mycobacteria, resistance to a compound containing a heavy metal.
A mycobacterium which carries in its chromosome a transposon comprising (1) at least one gene coding for resistance to a compound containing a heavy metal, and (2) a gene coding for an antigen.
Dependencies are inferred from the claim text.