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US 6,072,050 AGranted
Synthetic promoters
AssigneePioneer Hi-Bred International, Inc.
InventorsBenjamin A. Bowen, Wesley B. Bruce, Guihua Lu, Lynne E. Sims, Laura A. Tagliani
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AssigneePioneer Hi-Bred International, Inc.
InventorsBenjamin A. Bowen, Wesley B. Bruce, Guihua Lu, Lynne E. Sims, Laura A. Tagliani
Estimating the term…
Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a "TATA to start" sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.
A synthetic DNA promoter sequence functional in a plant cell, said promoter sequence comprising a TATA motif,
A synthetic DNA promoter sequence functional in a plant cell, said promoter sequence comprising a TATA motif,
An expression cassette comprising a synthetic promoter comprising a TATA motif,
An expression cassette comprising a synthetic promoter comprising a TATA motif,
An expression cassette comprising a synthetic promoter comprising a TATA motif,
A synthetic upstream element having a sequence set forth in SEQ ID NO:2.
An expression cassette comprising:
An isolated nucleotide sequence comprising a DNA enhancer sequence comprising the nucleotide sequence set forth in SEQ ID No:
A nucleotide sequence comprising a promoter construct, said construct comprising in operable linkage a core promoter sequence and a Ubi-1 UAR, wherein said Ubi-1 UAR is a maize Ubi UAR comprising the sequence set forth in SEQ ID No:
An expression cassette comprising in operable linkage a core promoter sequence,
Dependencies are inferred from the claim text.