# US 6,071,520 A · Chicken anemia virus mutants and vaccines and uses based on the viral proteins VP1, VP2 and VP3 or sequences of that virus coding therefor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 6,071,520 A - Document ID: US6071520A - Status: Granted - Kind: A - Application number: 08/454,121 - Filed: 1995-11-30 - Granted: 2000-06-06 - Earliest claimed: 1990-09-12 - Assignees: Leadd B.V. - Inventors: Mathieu Hubertus Maria Noteborn; Guus Koch - Web page: https://patentgrep.com/patent/US6071520A ## CPC - C07K 14/005: from viruses - A61P 31/12: Antivirals - C12N 15/86: Viral vectors - G01N 33/56983: {Viruses} - A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) - A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53) - C12N 2710/14143: viral genome or elements thereof as genetic vector - C12N 2750/12022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes - C12N 2750/12034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) ## Abstract Novel proteins of the Chicken Anemia Virus are described and compositions for preventing or treating infections with that virus (CAV), in particular vaccines less pathogenic than the CAV itself, but yet leading to neutralizing antibodies in the immunized animal. Besides, there are described compositions containing antibodies against parts of the CAV for the control of infections with CAV and anti-idiotype antibodies. The invention also provides antibodies and test kits for the detection of CAV. Recombinant DNA molecules derived from CAV and host cells transfected therewith and vaccines based on these host cells are made possible by this invention. The invention also comprises living virus vaccines in which a piece of DNA is brought into a virus infectious to the host. Besides, the invention provides uses of proteins of CAV in the induction of apoptosis, in particular in tumor cells. It further provides the induction of cell death by means of gene therapy. ## Claims ### Claim 1 A composition for generating antibodies against a Chicken Anemia Virus ("CAV"), said composition comprising: a first polypeptide comprising an amino acid sequence depicted in SEQ ID NO: 5, a second polypeptide comprising an amino acid sequence depicted in SEQ ID NO: 3, and an adjuvant in an amount sufficient to generate antibodies against a CAV when said composition is introduced into an animal. ### Claim 2 (depends on 1) The composition according to claim 1, wherein said antibodies are neutralizing antibodies. ### Claim 3 (depends on 1) The composition according to claim 1, wherein said animal is a chicken. ### Claim 4 (depends on 3) The composition according to claim 3, wherein said animal is a mouse. ### Claim 5 A method of generating antibodies against a Chicken Anemia Virus ("CAV") polypeptide, said method comprising: providing an animal with a sufficient amount of a composition which elicits an immune response in said animal, wherein said composition comprises a first polypeptide comprising an amino acid sequence depicted in SEQ ID NO: 5, and a second polypeptide comprising an amino acid sequence depicted in SEQ ID NO: 3, whereby antibodies against said CAV polypeptide are generated. ### Claim 6 (depends on 5) The method according to claim 5, wherein said composition further comprises an adjuvant. ### Claim 7 (depends on 5) The method according to claim 5, wherein said animal is a chicken. ### Claim 8 (depends on 5) The method according to claim 5, wherein said animal is a mouse.