Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

A method of manipulating biological samples comprising a mixture of desired cellular materials and undesired cellular materials, the manipulation being achieved in a dielectrophoretic system including a flow chamber having an array of electrodes, comprising the steps of:

• introducing a cell mixture suspension into the flow chamber of the system;
• subjecting the cell mixture suspension to an electric field by biasing the electrodes in a checkerboard format such that desired cells collect at the electrodes and undesired cells collect between the electrodes;
• and
• washing the undesired cells out of the flow chamber.

A method of manipulating biological samples comprising a mixture of desired cellular materials and undesired cellular materials, the manipulation being achieved in a dielectrophoretic system including a flow chamber having a first array of electrodes for separating and lysing, and a second array of electrodes which include oligonucleotide capture probes for hybridizing, comprising the steps of:

• introducing a cell mixture suspension into the flow chamber of the system;
• subjecting the cell mixture suspension to an electric field by biasing the first array of electrodes in a checkerboard format such that desired cells collect at the electrodes and undesired cells collect between the electrodes;
• washing the undesired cells out of the flow chamber;
• lysing the desired cells retained in the flow chamber of the system by subjecting the cells to a series of electronic pulses having alternating polarity;
• subjecting the lysed material in the flow chamber of the system to an enzymatic reaction;
• and
• hybridizing the enzymatic reaction product by exposure to the second array of electrodes.

A method of manipulating biological samples comprising a mixture of desired cellular materials and undesired cellular materials, the manipulation being achieved in a dielectrophoretic system including a flow chamber having a first array of electrodes for separating and lysing, and a second array of electrodes which include oligonucleotide capture probes for hybridizing, comprising the steps of:

• introducing a cell mixture suspension into the flow chamber of the system;
• subjecting the cell mixture suspension to an electric field by biasing the first array of electrodes in a checkerboard format such that desired cells collect at the electrodes and undesired cells collect between the electrodes;
• washing the undesired cells out of the flow chamber;
• lysing the desired cells retained in the flow chamber of the system by subjecting the cells to a series of electronic pulses having alternating polarity;
• subjecting the lysed material in the flow chamber of the system to an enzymatic reaction;
• and
• exposing the enzymatic reaction product to the second array of electrodes for ligand binding.