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US 6,066,484 AGranted
Purine nucleosidase
AssigneeSuntory Limited
InventorsHaruyo Hatanaka, Toshihiko Ashikari, Jun Ogawa, Sakayu Shimizu
Life of this patent
Estimating the term…
Abstract
Novel purine nucleosidase derived from Ochrobactrum anthropi microorganisms, a gene system coding therefor, uses therefor and particularly its use in the production of beer.
Claims2
A purified purine nucleosidase with the following enzymological properties:
- (1) substrate specificity:
- acts on purine compounds;
- (2) optimum pH:
- pH 5.0 to 7.5 for adenosine, pH 4.0 to 5.5 for guanosine, and pH 5.5 for inosine as substrates;
- (3) pH stability:
- exhibits at least 90% residual activity at pH 6.5 to 7.0 with treatment at 50.degree. C. for 60 minutes and exhibits at least 80% residual activity at pH 6.0 to 7.0 with treatment at 30.degree. C. for 30 minutes;
- (4) optimum temperature:
- optimum temperature of 60.degree. C. with adenosine or inosine substrate, optimum temperature of 50.degree. C. with guanosine substrate;
- (5) temperature stability:
- for adenosine as substrate, stability exhibited up to 40.degree. C. with treatment at pH 6.0 for 30 minutes and up to 30.degree. C. with treatment at pH 4.5 for 60 minutes;
- (6) molecular weight:
- 172,000 (measured by gel filtration chromoatography), 43,000 (subunits, measured by SDS-polyacrylamide gel electrophoresis).
9 more elements
A purified purine nucleosidase protein having an amino acid sequence from amino acid number 1 to amino acid number 341 of SEQ ID NO:2.
Dependencies are inferred from the claim text.