Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
AssigneeApplied Spectral Imaging Ltd.
InventorsYuval Garini, Dario Cabib, Robert A. Buckwald, Thomas Ried, Dirk G. Soenksen
Life of this patent
Estimating the term…
Abstract
A spectral imaging method for simultaneous detection of multiple fluorophores aimed at detecting and analyzing fluorescent in situ hybridizations employing numerous chromosome paints and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores for color karyotyping, and at multicolor chromosome banding, wherein each chromosome acquires a specifying banding pattern, which pattern is established using groups of chromosome fragments labeled with various fluorophore or combinations of fluorophores.
Claims13
A method of hybridization based multicolor chromosome banding comprising the steps of:
- (a) providing a metaphase chromosome spread of a first species and chromosome paints of a second species, at least some of said chromosome paints being labeled with a different fluorophore or combination of fluorophores;
- (b) hybridizing said chromosome paints with said metaphase chromosome spread;
- (c) using a fluorescence microscope being optically coupled to a multiband collection device for collecting spectral data from said chromosome spread;
- and
- (d) based on said collected data calculating a color image in which pixels associated with each of said different fluorophores or combination of fluorophores are attributed distinctive colors, thereby a banded color image of said metaphase chromosome spread is obtainable;
- wherein said method is for an application selected from the group consisting of providing a color banding karyotype of embryonic cells, providing a color banding karotype of white blood cells, providing a color banding karyotype of malignant cells, providing a color banding karyotype of cells examined for malignancy, detecting the stage of a malignancy, following the efficiency of an anti-cancer treatment and detecting chromosomal aberrations following exposure to mitogenic agents.
Dependencies are inferred from the claim text.