Immunoassay for homocysteine
AssigneeAxis-Shield ASA
InventorErling Sundrehagen
Life of this patent
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Abstract
The invention relates to a method for assaying homocysteine in a sample such as blood, plasma or urine, which comprises the steps of contacting the sample with a homocysteine converting enzyme and at least one substrate for the enzyme other than homocysteine, and without chromatographic separation, assessing a non-labelled analyte selected from a homocysteine co-substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme.
Claims29
An immunological method for indirectly assaying homocysteine in a sample, said method comprising the steps of:
- (a) contacting said sample with S-adenosyl homocysteine hydrolase enzyme and adenosine or an analogue of adenosine, wherein said S-adenosyl homocysteine hydrolase converts said homocysteine into a non-labelled analyte, wherein said non-labelled analyte is S-adenosyl homocysteine or an analogue thereof;
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- (b) determining the presence or amount of the non-labelled analyte without chromatographic separation by contacting said sample with an antibody which specifically binds with said non-labelled analyte and with a detectable hapten for said antibody other than said non-labelled analyte, and wherein determining the presence or amount of said non-labelled analyte is effected indirectly by determining the presence or amount of said detectable hapten either bound or not bound to said antibody, wherein the amount of the non-labelled analyte is directly proportional to the amount of homocysteine in said sample.
An immunological method for indirectly assaying homocysteine in a sample, said method comprising the steps of:
- (a) contacting said sample with S-adenosyl homocysteine hydrolase enzyme and S-adenosyl homocysteine wherein said S-adenosyl homocysteine hydrolase converts said S-adenosyl-homocysteine into a non-labelled analyte wherein said non-labelled analyte is adenosine;
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- (b) determining the presence or amount of the non-labelled analyte without chromatographic separation by contacting said sample with an antibody which specifically binds with said non-labelled analyte and with a detectable hapten for said antibody other than said non-labelled analyte and wherein determining the presence or amount of said non-labelled analvte is effected indirectly by determining the presence or amount of said detectable hapten either bound or not bound to said antibody, wherein the amount of the non-labelled analyte is indirectly proportional to the amount of homocysteine in said sample.
Dependencies are inferred from the claim text.