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US 6,057,287 AGranted
Kallikrein-binding "Kunitz domain" proteins and analogues thereof
AssigneeDyax Corp.
InventorsWilliam Markland, Robert Charles Ladner
Life of this patent
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Abstract
This invention relates to Kunitz domain proteins that bind to, and preferably inhibit, one or more kallikreins, and to therapeutic, diagnostic, and purification use of these proteins.
Claims8
A kallikrein binding protein of 56-60 amino acids comprising a non-naturally occurring Kunitz domain, said Kunitz domain having disulfide bonds at Cys5-Cys55, Cys14-Cys38, and Cys30-Cys51 and further having:
- amino acid number 13 selected from His and Pro;
- amino acid number 14 Cys;
- amino acid number 16 selected from Ala and Gly;
- amino acid number 17 selected from Ala, Asn, and Ser;
- amino acid number 18 selected from His and Leu;
- and
- amino acid number 19 selected from Gln, Leu, and Pro,
- wherein the above amino acids are numbered to correspond to the numbering of amino acid residues of bovine pancreatic trypsin inhibitor (BPTI) (SEQ ID NO:1).
5 more elements
A kallikrein binding protein of 56-60 amino acids comprising a non-naturally occurring Kunitz domain, said Kunitz domain having disulfide bonds at Cys5-Cys55, Cys14-Cys38, and Cys30-Cys51 and further having:
- amino acid number 13 selected from His and Pro;
- amino acid number 14 Cys;
- amino acid number 15 selected from Lys and Arg;
- amino acid number 16 selected from Ala and Gly;
- amino acid number 17 selected from Ala, Asn, and Ser;
- amino acid number 18 selected from His and Leu,
- amino acid number 19 selected from Gln, Leu, and Pro;
- amino acid number 31 is Glu;
- amino acid number 32 selected from Glu and Gln;
- amino acid number 34 selected from Ser, Thr, and Ile;
- and
- amino acid number 39 selected from Gly, Glu, and Ala,
- wherein the above amino acids are numbered to correspond to the numbering of amino acid residues of bovine pancreatic trypsin inhibitor (BPTI) (SEQ ID NO:1).
10 more elements
Dependencies are inferred from the claim text.