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AssigneeKikkoman Corporation
InventorsHiroki Tatsumi, Naoki Eisaki, Tatsuo Horiuchi, Ayumu Nagahara
Estimating the term…
Disclosed are a pyruvate orthophosphate dikinase gene coding for a polypeptide containing the amino acid sequence of SEQ ID NO:1 or an amino acid sequence where in said amino acid sequence one or more amino acids are added, deleted or substituted and bringing about the activity of pyruvate orthophosphate dikinase; recombinant DNA having the pyruvate orthophosphate dikinase gene integrated in vector DNA; and a process for producing pyruvate orthophosphate dikinase, which comprises culturing a microorganism belonging to the genus Escherichia carrying the above recombinant DNA in a medium, and recovering pyruvate orthophosphate dikinase from the resulting culture. The pyruvate orthophosphate dikinase gene and a process for producing pyruvate orthophosphate dikinase are provided.
An isolated and purified nucleic acid comprising at least a portion of a nucleotide sequence which encodes SEQ ID NO:2, and wherein said nucleotide sequence encodes a pyruvate orthophosphate dikinase.
An isolated and purified nucleic acid having a nucleotide sequence which encodes SEQ ID NO:2.
A method for producing pyruvate orthophosphate dikinase, comprising:
A method for producing pyruvate orthophosphate dikinase, comprising:
Dependencies are inferred from the claim text.