Method of amplifying the signal of target nucleic acid sequence analyte

A method of amplifying the signal of target nucleic acid sequence analyte, comprising the steps of:

• (1) hybridizing a first end of a bidirectional primer to a circular DNA template;
• (2) polymerizing at said hybridized first end of said bidirectional primer using said circular DNA template in the presence of a multiplicity of signal amplification sequence units to form a complementary nucleic acid sequence of said target nucleic acid sequence to which are covalently attached said repeating signal amplification sequence units;
• (3) hybridizing a second end of said bidirectional primer to a target nucleic acid sequence;
• and
• (4) polymerizing at said hybridized second end of said bidirectional primer to form a complementary nucleic acid sequence of said target nucleic acid sequence,
• wherein said bidirectional primer contains a first end complementary to said circular DNA template and a second end complementary to said target nucleic acid sequence.

An array for detecting target nucleic acid sequences, comprising:

• a. a substrate having a surface;
• b. a multiplicity of capture probes attached to said surface of said substrate;
• and
• c. a multiplicity of complementary nucleic acid sequences of said target nucleic acid sequences to which are covalently attached repeating signal amplification sequence units.