Enzyme sensor

A membrane for an enzyme sensor for measuring the concentration or activity of an analyte in a test fluid, said membrane having at least one enzyme layer comprising an immobilized enzyme for which the analyte is a substrate, the immobilized enzyme being obtained by formation of one or more covalent link(s), optionally by using a cross-linking agent, between the enzyme and at least one type of macromolecule in the presence of a competitive inhibitor for said enzyme.

A method for improving the stability of the enzymatic activity of an immobilized enzyme included in an enzyme sensor for measuring the concentration of an analyte in a test fluid, the analyte being a substrate for said enzyme, the method comprising intermittently or continuously exposing the immobilized enzyme to a solution comprising a competitive inhibitor for said enzyme.

A stabilizing agent for an immobilized enzyme included in an enzyme sensor of a solution comprising a competitive inhibitor for said enzyme wherein the enzyme sensor is adapted for measuring the concentration or activity of an analyte in a test fluid, the analyte being a substrate for the enzyme.

An apparatus for measuring the concentration or activity of one or more analyte(s) in a test fluid, the apparatus comprising an enzyme sensor having at least one layer of an immobilized enzyme for which at least one of the analytes is a substrate, wherein the apparatus further comprises one or more containers with solution(s) needed for the operation of the apparatus, at least one solution comprising a competitive inhibitor for the enzyme, and means for intermittently exposing the sensor to the solution comprising the competitive inhibitor.